An Escherichia coli O157:H7 dps::nptI mutant (FRIK 47991) was generated, and its survival was compared to that of the parent in HCl (synthetic gastric fluid, pH 1.8) and hydrogen peroxide (15 mM) challenges. The survival of the mutant in log phase (5-h culture) was significantly impaired (4-log(10)-CFU/ml reduction) compared to that of the parent strain (ca. 1.0-log(10)-CFU/ml reduction) after ...

A variant H-1 virus, designated H-1 dr virus, was isolated from stock of the standard H-1 virus strain propagated in the newborn human kidney cell line NB-E. Molecular cloning and sequence analysis revealed an in-frame deletion at map positions 39 to 41. This deletion affects the open reading frames encoding the nonstructural proteins NS-1 and NS-2 and the untranslated leader sequence of the R3...

Wild-type measles virus (MV) isolated in B95a cells could be adapted to Vero cells after several blind passages. In this study, we have determined the complete nucleotide sequences of the genomes of the wild type (T11wild) and its Vero cell-adapted (T11Ve-23) MV strain and identified amino acid substitutions R516G, E271K, D439E and G464W (D439E/G464W), N481Y/H495R, and Y187H/L204F in the nucleo...

Previous experimental and computational results have confirmed that the thermal conductivity of a two-dimensional (2D) material can be considerably affected by strain. Numerous attention has been paid to explore relevant mechanisms. However, strain effects on interfacial conductance (ITC) 2D heterostructure attracted little attention. Herein, non-equilibrium molecular dynamics (NEMD) simulation...

Pharmacodynamic activity in antibiotic combinations of daptomycin, vancomycin, and linezolid was investigated in a 48-h in vitro pharmacodynamic model. Using human-simulated free drug concentrations, activity against clinical biofilm-forming methicillin-resistant Staphylococcus aureus isolates was evaluated. Linezolid antagonized vancomycin activity at 24 and 48 h. Linezolid antagonized daptomy...

s of papers 41 (H=79.9 vs. I=81.0 eggs), and M (H=5.4 vs. I=20.9%). Also, strain effected (p<.001) bone BS (H=14.26 vs. I=12.26 kg) and volume (H=5.90 vs. I=6.27 cc). These results indicate that strain influences performance and bone parameters and suggest that increased Ca and P levels impact bones of laying hens only if provided during the rearing phase.

This study reports a non-destructive method of measuring the residual strain in the GaN epilayer grown on sapphire substrate by micro-Raman spectroscopy. Operating in confocal mode this method allows a depth-dependent measurement of residual strain in the epitaxial layer without prior treatment of the sample. This approach to measurement of residual strain is demonstrated on GaN epitaxial films...

T he whole genome sequence of Helicobacter pylori strain 26695 was published in 1997 and that of a second strain, J99, in 1999. 2 These publications moved H pylori research forward by a quantum leap. Comparing H pylori genes with genes of known function in other bacteria gave immediate insights into H pylori metabolism, structure, adaptive mechanisms, and virulence. Research became more focused...

Wheel-running activity patterns were studied in two inbred rat strains (ACI/Ztm and LEW/Ztm) under 24-h light-dark cycles with various photoperiods. The ACI strain was characterized by a unimodal activity pattern, whereas the LEW strain exhibited a multimodal activity pattern consisting of two activity bouts about 3-5 h apart. Harmonic spectral analyses and chi square periodograms revealed stra...

Most Helicobacter pylori strains are susceptible to amoxicillin, an important component of combination therapies for H. pylori eradication. The isolation and initial characterization of the first reported stable amoxicillin-resistant clinical H. pylori isolate (the Hardenberg strain) have been published previously, but the underlying resistance mechanism was not described. Here we present evide...