Production of cellulosic ethanol from lignocellulosic biomass leads to the generation a hemicellulosic hydrolysate during feedstock pretreatment. This is rich in sugars, but also contains inhibitory compounds (mainly acetic acid and phenolic compounds) concentrations that may be toxic microbial growth. Currently, this side-stream production process processed as waste, due lack feasible alternat...

We have studied the significance of the N-terminal presequence of watermelon (Citrullus vulgaris) glyoxysomal malate dehydrogenase [gMDH; (S)-malate:NAD+ oxidoreductase; EC 1.1.1.37] in microbody targeting. The yeast Hansenula polymorpha was used as heterologous host for the in vivo expression of various genetically altered watermelon MDH genes, whose protein products were localized by immunocy...

In yeasts and mammals, PEX10 encodes an integral membrane protein with a C3HC4 RING finger motif in its C-terminal domain and is required for peroxisome biogenesis and matrix protein import. In humans, its dysfunction in peroxisome biogenesis leads to severe Zellweger Syndrome and infantile Refsum disease. Here we show that dysfunction of a homologous gene in Arabidopsis leads to lethality at t...