In this work, the first systematic characterization of triacylglycerol (TG) enantiomers in real samples using chiral high-performance liquid chromatography (HPLC) with atmospheric pressure chemical ionization mass spectrometry (APCI-MS) is performed. Our chiral HPLC/APCI-MS method is based on the use of two cellulose-tris-(3,5-dimethylphenylcarbamate) columns connected in series using a gradien...

BACKGROUND The double-blind, placebo-controlled food challenge (DBPCFC) is considered the definitive diagnostic test for food allergy. Nevertheless, validated recipes for masking the foods are scarce, have not been standardized, and differ between centers. Sensory evaluation techniques such as the triangle test are necessary to validate the recipes used for DBPCFC. METHODS We developed 3 reci...

Hazelnut (Corylus avellana L.) is a popular tree nut worldwide, mainly distributed along the coasts of the Black Sea region of Turkey, southern Europe (Italy, Spain, Portugal, and France), and in some areas of the US (Oregon and Washington). Turkey is the world’s largest producer of hazelnuts, contributing ~74% to the total global production. In this study, compositional characteristics of eigh...

Hazelnuts are rich in monounsaturated fatty acids and antioxidant bioactive substances: their consumption has been associated with a decreased risk of cardiovascular disease events. A systematic review and a meta-analysis was performed to combine the results from several trials and to estimate the pooled (overall) effect of hazelnuts on blood lipids and body weight outcomes. Specifically, a Bay...

Thermal inactivation kinetics (D and z values) of Salmonella Enterococcus faecium in fresh hazelnut shell kernel were determined. Aluminum pouches containing either 1.5 g powder or 2 ground inoculated with bacterium at their original water activities (aw-shell = 0.91 ± 0.01, aw-kernel 0.93 0.01) isothermally treated a thermal death time (TDT) sandwich 55, 60, 65, 70 75 °C for determining D valu...

Honey is a natural product with many attributes that are useful for humans. The consumption of honey is increasing because of its beneficial biological properties, including antioxidant and antibacterial activities. Even though honey is produced worldwide, many variants of this product have not been studied and their biological potential for alternative uses has not been evaluated. Because of i...

Nonspecific lipid transfer proteins (nsLTPs) are basic proteins, stabilized by four disulfide bonds, and are expressed throughout the plant kingdom. These proteins are also known as important allergens in fruits and tree nuts. In this study, the nsLTP from hazelnuts, Cor a 8, was purified and its crystal structure determined. The protein is stable at low pH and refolds after thermal denaturatio...