Objective Adiponectin and its receptors (AdipoR1 and AdipoR2), known as adiponectin system, have some proven roles in the fat and glucose metabolisms. Several studies have shown that adiponectin can be considered as a candidate in linking metabolism to testicular function. In this regard, we evaluated the correlation between sperm mRNA abundance of adiponectin and its receptors, with sperm moti...

Background: Formaldehyde (FA, H2CO), a member of the aldehyde family and one of the simplest organic molecules, is a flammable, pungent, irritating and colorless gas. This study aimed to investigate the effect of formaldehyde (FA) vapor on sperm parameters and testicular tissue concern about the duration of analysis and the amount of the formaldehyde vapor concentrations. Materials and Methods:...

Parous ewes were treated orally with medroxyprogesterone acetate (MAP) or melengestrol acetate (MGA), subcutaneously with progesterone, or intra-vaginally with MAP, MGA or progesterone. Treatment was begun on day 8 of an estrous cycle and continued for 17 days. When the ewes were in estrus after progestagen withdrawal, they were inseminated artitlcally and their vulvo-vaginal junctions were lig...

The presence and role of c-ras proteins were investigated in mature human sperm cells. The v-H-ras monoclonal antibody (mAb) against the c-ras protein, p21, reacted specifically with the acrosomal region of methanol-fixed as well as unfixed-live capacitated and non-capacitated human sperm cell in the indirect immunofluorescence technique. The v-H-ras mAb predominantly recognized c-ras protein o...

background: nitric oxide (no) that plays important role in all sexual activities of animals is made from the amino acid l-arginine by the enzymatic action of no synthase (nos). no makes a band with sulfur-iron complexes, but due to production of steroid sexual hormones related to the enzymes involved in this complex, no can change the activity of these enzymes. no affects many cells including v...

Here we describe an improved method of separating sperm cells from lysed epithelial cells via acoustic cell trapping. In particular, we show that in the processing of samples with low numbers of sperm cells, addition of polymeric beads to the sample[1] allowed for better performance in terms of higher trapping efficiency versus our previous acoustic differential extraction (ADE) results[2]. We ...

The normal structure and function of sperm are prerequisites for successful fertilization and embryonic development, but little is known about how defective sperm are eliminated during mammalian spermatogenesis. Here, we describe a ubiquitin-dependent, sperm quality control mechanism that resides in the mammalian epididymis, the site of sperm maturation and storage. We used immunofluorescence, ...

Sterile backcross (Be) males originate from the hybridization of Heliothis subflexa (Guennee) females to H. virescens (F.) males followed by recurrent backcrossing of the fertile female progeny to H. virescens males. Ultrastructural transmission electron microscope (TEM) studies of the postmeiotic maturation of eupyrene sperm cells in Be males, and comparisons with the cells in normal H. viresc...

Seminal fluids are known to have a variety of effects on rival sperm, but in externally fertilizing species it is still unclear what effects seminal fluid can induce under sperm competition. We recorded sperm activity from natural ejaculates (including own seminal fluid) of an external fertilizer, the Arctic charr (Salvelinus alpinus), after activation either in water (the natural medium for mi...

objective: epididymo-orchitis (eo) potentially results in reduced fertility in up to 60% of affected patients. the anti-inflammatory effects of korean red ginseng (krg) and its ability to act as an immunoenhancer in parallel with the beneficial effects of this ancient herbal medicine on the reproductive systems of animals and humans led us to evaluate its protective effects against acute eo. ma...