The cumulative stressors of exercise manifest themselves at a cellular level by threatening the protein homeostasis of the cell. In these conditions, Heat Shock Proteins (HSP) are synthesised to chaperone mis-folded and denatured proteins. As such, the intracellular HSP response is thought to aid cell survival in the face of otherwise lethal cellular stress. Recently, the inducible isoform of t...

Stress tolerance of Saccharomyces cerevisiae was examined after exposure to heat and salt shock in the presence or absence of the protein synthesis inhibitor cycloheximide. Cells heat-shocked (37 degrees C for 45 min) in the absence of cycloheximide demonstrated increased tolerance of heat, freezing and salt stress. For cells heat-shocked in the presence of cycloheximide, heat and salt toleranc...

Heat shock factor 1 (HSF1) is a heat shock transcription factor that rapidly induces heat shock gene transcription following thermal stress. In this study, we subjected primary neonatal rat myocardial cells to heat stress in vitro to create a model system for investigating the trends in expression and association between various heat shock proteins (HSPs) and HSF1 under adverse environmental co...

Ambient temperature is one of the most important environmental factors affecting physiological mechanisms and biochemical reactions of living organisms. Thus the effect of ambient temperature on HSPs and IGF-I gene expression levels in the liver and muscle tissues of Sparus aurata were investigated in this research. The levels of HSPs, and IGF-I gene expression of the liver and muscle of Sparus...

In vivo protein expression in the abdominal viscera of C. v. slmorensis was examined from aduh flies that were cold shocked for various lengths of time at O, 10, or 15°C and labelled at 25°C with 35S-methionine at 0, 2, 4 and 6 hr during the recovery period. In vitro labelling showed that seven unique proteins (23, 40, 43, 48, 60, 70 and 92 kDa) were produced in C. v. sonorensis exposed to low ...

This study evaluated associations of heat shock proteins (HSP) and an oxidative stress protein, protein deglycase (DJ1), with beef quality tenderness. Samples from the longissimus thoracis (N = 99) were collected pre-rigor (day 0) after 14-d aging. Warner-Bratzler shear force (WBSF), myofibrillar fragmentation index (MFI), a trained sensory panel used to determine meat quality. Protein abundanc...

Heat shock proteins (HSPs) constitute an endogenous cellular defense mechanism against environmental stresses. In the past few years, studies have shown that overexpression of HSPs can protect cardiac myocytes against ischemia-reperfusion injury. In an attempt to increase the HSPs in cardiac tissue, we used the compound radicicol that activates HSP expression by binding to the HSP 90 kDa (HSP90...

The synthesis of heat shock proteins (HSP) and the development of thermotolerance were studied in Chinese hamster ovary cells in order to determine whether depletion of intracellular glutathione (GSH) inhibited their expression. Cells were exposed to 100 MMdiethylmaleate/50 H.M buthionine sulfoximine which reduced GSH levels by 95% or more during the experimental time course. HSP synthesis was ...

We have for the first time characterized the heat shock response in mycobacteria both at the level of transcription, by RNA extraction, Northern (RNA) blotting, and hybridization with gene-specific probes for the Mycobacterium tuberculosis 65- and 71-kDa heat shock proteins (HSPs), and at the level of translation, by [35S]methionine labelling, sodium dodecyl sulfate-polyacrylamide gel electroph...

Modulation of the host immune system represents a promising therapeutic approach against cancer, including multiple myeloma. Recent findings indicate that the NK group 2D (NKG2D)- and DNAX accessory molecule-1 (DNAM-1)-activating receptors play a prominent role in tumor recognition and elimination by cytotoxic lymphocytes, suggesting that the levels of NKG2D and DNAM-1 ligand expression on tumo...