By taking advantage of cellulose, graphene oxide (GO), and the process for crosslinking using epichlorohydrin (ECH), we propose a simple and novel method to prepare GO/cellulose hydrogel with good potential to adsorb metal ions. GO nanosheets containing carboxyl and hydroxyl groups were introduced into the surface of the cellulose hydrogel with retention of the gel structure and its nanoporous ...

The literature on inhibitors of hyaluronidase is both voluminous and contradictory. The contradictions are caused by the differences in methods used, by the differences in enzyme purity, and to a very high degree by the differences in the purity of the substrates used. The latter are often contaminated with protein, with glycogen, and with mucopolysaccharides other than hyaluronic acid, especia...

The effects of the heavy-metal ions Cd2+ and Zn2+ on the homoeostasis of intracellular free Ca2+ in E367 neuroblastoma cells were examined using 19F-NMR spectroscopy with the fluorinated chelator probe 1,2-bis-(2-amino-5-fluorophenoxy)ethane-N,N,N', N'-tetra-acetic acid (5F-BAPTA). First, the technique was used to quantify the uptake and intracellular free concentrations of the heavy metals aft...

A novel SmtB/ArsR family metalloregulator, denoted BxmR, has been identified and characterized from the cyanobacterium Oscillatoria brevis. Genetic and biochemical evidence reveals that BxmR represses the expression of both bxa1, encoding a CPx-ATPase metal transporter, as well as a divergently transcribed operon encoding bxmR and bmtA, a heavy metal sequestering metallothionein. Derepression o...

The viability of base-treated juniper fiber (BTJF) media for removing toxic heavy metals (Cd2+, Cu2+, Pb2+, Zn+) in stormwater runoff was investigated. The sorption ability ofthe BTJF for all metals was much higher than that of untreated juniper. The affinity sequence of both materials, BTJF and untreated juniper, was Pb > Cu > Zn:,. Cd. This order is explained by the hydrolysis constants for e...

Many coal-fired power plants are implementing ammonia-based technologies to reduce NO(x) emissions. Excess ammonia in the flue gas often deposits on the coal fly ash. Ammonia can form complexes with many heavy metals and change the leaching characteristics of these metals. This research tends to develop a fundamental understanding of the ammonia impact on the leaching of some heavy metals, exem...

Most marine bacteria can produce exopolysaccharides (EPS). However, very few structures of EPS produced by marine bacteria have been determined. The characterization of EPS structure is important for the elucidation of their biological functions and ecological roles. In this study, the structure of EPS produced by a marine bacterium, Alteromonas sp. JL2810, was characterized, and the biosorptio...

In this Application Note, separation matrices for protein characterization by capillary gel electrophoresis (CGE) with UV detection from two suppliers, Beckman Coulter and Advanced Analytical, were compared on the Agilent 7100 CE system. Two different sample sets were analyzed: fi rst, a protein size standard and BSA as a test protein for molecular weight determination; second, a reduced antibo...

A copper(II) ion-selective PVC membrane sensor based on 2-(1'-(4'-(1''-hydroxy-2''-naphthyl)methyleneamino)butyl iminomethyl)-1-naphthol (BHNB) as a novel Schiff base containing a sensing material has been successfully developed. The sensor exhibits a good linear response of 29 mV per decade within the concentration range of 10(-1)-10(-6) M of Cu2+. The sensor shows good selectivity for copper(...