OBJECTIVE To characterize alpha-chain variant hemoglobins with electric mobility similar to that of hemoglobin S in a newborn screening program. METHODS β(S) allele and alpha-thalassemia deletions were investigated in 14 children who had undefined hemoglobin at birth and an electrophoretic profile similar to that of hemoglobin S when they were six months old. Gene sequencing and restriction e...

3. Perrine SP, Greene MF, Failer DV. Delay in the fetal globin switch in infants of diabetic mothers. N Engl J Med 1985;312:334-8. 4. Bard H, Prosmanne J. Relative rates of fetal hemoglobin and adult hemoglobin' synthesis in cord blood of infants of insulin-dependent mothers. Pediatrics 1985;75:1143-7. 5. Bard H, Prosmanne J. Postnatal fetal and adult hemoglobin synthesis in preterm infants who...

Three embryonic hemoglobins are formed in erythroid cells derived from yolk sac blood islands in fetal mice of the C57BL/6J strain. These cells provide a suitable model to study the rates of synthesis of specific proteins during the differentiation of cells. Erythroid cells differentiate initially in yolk sac blood islands from the 8th to the 10th day of gestation. (Fetal mice have a ZOto Zl-da...

The spin label technique has been used to study human hemoglobins A, F, Zürich, and Chesapeake as a function of carbon monoxide saturation. The experimental results suggest that the changes in the electron paramagnetic resonance spectra of hemoglobin labeled with N-(1-oxyl-2,2,6,6-tetramethyl-4-piperidinyl)iodoacetamide depend on the state of ligation of more than one heme group. For those hemo...

Erythropoiesis in the mouse begins with the production of a distinct population of red blood cells in the blood islands of the visceral yolk sac at day 7.5 postcoitum (p.c.). These primitive yolk sac red blood cells are large (about four times the volume of adult erythrocytes), nucleated and produce a distinctive set of embryonic hemoglobins (Craig and Russell, 1964; Russell and Bernstein, 1966...

In the course of the study on the hemoglobin contained in newborn bovine blood, two major and five minor components which are specific to newborn bovine hemoglobin were detected by the methods of agar-gel electrophoresis, cellulose-acetate membrane electrophoresis and column chromatography. These components were examined on their alkaline resistance , and the results showed that all the compone...

FREE-BOTJNDARY ELECTROPHORESI8 has been used for the isolation, from fresh extracts of normal human red blood cells, of a hemoglobin, which at pH 8.8 was more negatively charged than the chief component (1) and which, on spectroscopic examination, has been found indistinguishable from the bulk of the oxyhemoglobin as regards the visible region and the Soret band (2). The proportion of this hemo...