UNLABELLED BACKGROUND There is no convenient cheap pragmatic experimental model for Nonalcoholic Fatty Liver Disease (NAFLD)/Nonalcoholic Steatohepatitis (NASH). OBJECTIVE Our objective was to create a pragmatic model of NAFLD/NASH. METHODS Sprague-Dawley rats were fed a high-fat, high sugar homemade diet ad libitum for seven weeks. The high-fat, high sugar diet included 59% of energy derived...

background : magnesium is the second most abundant intracellular cation. it plays an important role in insulin homeostasis and glucose metabolism through multiple enzymatic reactions. with increasing data on magnesium deficiency in diabetic patients and epidemiological studies demonstrating magnesium deficiency as a risk factor for diabetes, it is logical to search for its possible beneficial e...

Hepatic steatosis is a core feature of the metabolic syndrome and type 2 diabetes and leads to hepatic insulin resistance. Malonyl-CoA, generated by acetyl-CoA carboxylases 1 and 2 (Acc1 and Acc2), is a key regulator of both mitochondrial fatty acid oxidation and fat synthesis. We used a diet-induced rat model of nonalcoholic fatty liver disease (NAFLD) and hepatic insulin resistance to explore...

Epinephrine infusion into the portal vein produced rapid changes in the activities of certain hepatic, epididymal fat, and skeletal muscle glycolytic enzymes and fructose diphosphatase in the rat. Epinephrine produced a rapid increase in hepatic, epididymal fat, and skeletal muscle fructose diphosphatase activity. In contrast, epinephrine produced a rapid decrease in hepatic phosphofructokinase...

Hyperoxia contributes to lung injury in experimental animals and bronchopulmonary dysplasia (BPD) in preterm infants. Cytochrome P4501A (CYP1A) enzymes, which are regulated by the aryl hydrocarbon receptor (AhR), have been shown to attenuate hyperoxic lung injury in rodents. Omeprazole, a proton pump inhibitor, used in humans to treat gastric acid-related disorders, induces hepatic CYP1A in vit...

PURPOSE Warm hepatic ischemia-reperfusion (I/R) injury can lead to multiorgan dysfunction. The aim of the present study was to investigate whether acute liver I/R does affect the function and/or structure of remote organs such as lung, kidney, and heart via modulation of extracellular matrix remodelling. METHODS Male Sprague-Dawley rats were subjected to 30 min partial hepatic ischemia by cla...

We investigated the effects of obstructive cholestasis in different hepatic lobes by evaluating asymmetric dimethylarginine (ADMA) (a nitric oxide synthase inhibitor), protein methyltransferase (PRMT) and dimethylarginine dimethylaminohydrolase (DDAH) (enzymes involved, resp., in its synthesis and degradation), the cationic transporter (CAT), and metalloproteinase (MMP) activity. Sixteen male W...

Objective(s): Silver nanoparticles show anti-fungal properties, and is widely used in medicine. In this research, the impacts of silver nanoparticles on the hepatic functional tests and changes in liver tissues in adult male rats were investigated. Methods: In this experimental study, 28 adult male Wistar rats, each weighing approximately 180-220 g were divide...

3-Aminobenzanthrone (3-ABA) is a human metabolite of carcinogenic 3-nitrobenzanthrone (3-NBA), which occurs in diesel exhaust and air pollution. Understanding which cytochrome P450 (CYP) enzymes are involved in metabolic activation and/or detoxication of this toxicant is important in the assessment of an individual's susceptibility to this substance. The aim of this study was to evaluate the ef...

the objectives of this study were to determinethe interactions between two hepatic enzymes and some minerals in the liver of broiler chickens. the study was performed with male and female from 1 to 56 days of age of broiler chickens. malic acid was added to the water and offered to chickens freely from the start to the end of the experiment with constant concentration. the treatments consisted ...