نتایج جستجو برای: hepatocytes

تعداد نتایج: 21626  

Journal: :Journal of cell science 1981
E Roos I V Van de Pavert O P Middelkoop

MB 6A lymphosarcoma and TA3 mammary carcinoma cells have previously been shown to infiltrate from liver blood vessels, where they had been arrested, into the liver parenchyma. The same tumour cells were previously added to cultures of isolated hepatocytes, 24 h after their isolation. Both tumour cell types adhered to both dorsal and lateral surfaces of hepatocytes. The lymphosarcoma cells rapid...

2015
Melissa Baxter Sarah Withey Sean Harrison Charis-Patricia Segeritz Fang Zhang Rebecca Atkinson-Dell Cliff Rowe Dave T. Gerrard Rowena Sison-Young Roz Jenkins Joanne Henry Andrew A. Berry Lisa Mohamet Marie Best Stephen W. Fenwick Hassan Malik Neil R. Kitteringham Chris E. Goldring Karen Piper Hanley Ludovic Vallier Neil A. Hanley

BACKGROUND & AIMS Hepatocyte-like cells (HLCs), differentiated from pluripotent stem cells by the use of soluble factors, can model human liver function and toxicity. However, at present HLC maturity and whether any deficit represents a true fetal state or aberrant differentiation is unclear and compounded by comparison to potentially deteriorated adult hepatocytes. Therefore, we generated HLCs...

Journal: :Biochemical and biophysical research communications 2006
Kinji Asahina Miho Shiokawa Takuro Ueki Chihiro Yamasaki Akio Aratani Chise Tateno Katsutoshi Yoshizato

Adult rat liver contains a minor population of hepatocytes called small hepatocytes (SHs) that are smaller in size and show a higher replicative potential than conventional parenchymal hepatocytes (PHs). However, SHs have been hitherto characterized using a "SH-fraction" that was contaminated with PHs. In the present study, we isolated a PH-free SH-fraction from the adult rat liver using fluore...

Journal: :Cell structure and function 1991
M Inagaki K Ogawa

To optimize conditions for retroviral-mediated transfer of a recombinant gene to hepatocytes, the pMNSM-Tk-lacZ vector, which we had constructed to express the bacterial beta-galactosidase gene, was transduced to rat hepatocytes under various conditions, and the expression of beta-galactosidase activity was examined by cytochemical staining. Compared to the hepatocytes of adult rats, those of n...

Journal: :Scientia Pharmaceutica 2022

Cinnamaldehyde (CA) is a natural compound that has promising biological activity. The current study investigates the antitumor activity of CA in thioacetamide induced hepatocellular carcinoma (HCC) rats through targeting Wnt/?-catenin pathway and evaluates capability to relieve hepatocytes oxidative stress HCC-rat model. After 16 weeks HCC induction by (TAA), were treated for 7 consecutive with...

Journal: :Journal of pharmacological sciences 2004
Dong-Soon Im Hideaki Tomura Masayuki Tobo Koichi Sato Fumikazu Okajima

We previously reported that sphingosine 1-phosphate (S1P) induces inhibition of adenylyl cyclase and activation of phospholipase C via independent G protein-coupled receptors in adult rat hepatocytes. Although S1P activation of phospholipase C and subsequent increase of intracellular Ca(2+) concentration were enhanced during the primary culture of hepatocytes, S1P inhibition of adenylyl cyclase...

1999
SANJEEV GUPTA CHARLES E. ROGLER

Gupta, Sanjeev, and Charles E. Rogler. Lessons From Genetically Engineered Animal Models. VI. Liver repopulation systems and study of pathophysiological mechanisms in animals. Am. J. Physiol. 277 (Gastrointest. Liver Physiol. 40): G1097–G1102, 1999.—The ability to localize transplanted hepatocytes in the liver offers exciting new opportunities. Transplanted hepatocytes enter liver plates, form ...

Journal: :iranian journal of allergy, asthma and immunology 0
gholamhossein hassanshahi abdollah jafarzadeh zohreh ghorashi nazanin zia sheikholeslami alan james dickson

chemokines are classified in four distinct groups as cxc, cc, cx3c and c, depending on the presence or absence of a motif called elr (arg-leu-glu) before the first cysteine residue in their structure. cxc chemokines are also subdivided into elr + and elr - . increasing evidence has indicated the existence of a chemokine network in the liver which is involved in both physiological responses and,...

Journal: :Endocrinology 2007
Agueda Gonzalez-Rodriguez Jill E Clampit Oscar Escribano Manuel Benito Cristina M Rondinone Angela M Valverde

Protein tyrosine phosphatase 1B (PTP1B) is a negative regulator of insulin signaling and a therapeutic target for type 2 diabetes. The purpose of this study was to evaluate the differences in insulin sensitivity between neonate and adult hepatocytes lacking PTP1B. Immortalized neonatal hepatocytes and primary neonatal and adult hepatocytes have been generated from PTP1B(-/-) and wild-type mice....

Journal: :The Journal of biological chemistry 1978
C C Malbon S Li J N Fain

The ability of catecholamines and glucagon to stimulate glycogen phosphorylase activity was examined in hepatocytes isolated from euthyroid and hypothyroid fed female rats. Hypothyroidism did not alter the ability of either phenylephrine or epinephrine to activate glycogen phosphorylase. Activation of glycogen phosphorylase in response to a maximal concentration of isoproterenol, however, was 1...

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