the potential mycoherbicide alternaria alternata was isolated from one lesion on a leaf of amaranthus retroflexus which had been inoculated with conidia of the fungus. the isolate was cultured on v-8 agar medium. hyphal tip isolates (hti) were derived from young germ tubes of single spores and cultured on a semidefined culture medium with a c:n ratio of 15:1. amplified fragment length polymorph...
