32 Printz, R. L., Koch, S., Potter, L. R., O’Doherty, R. M., Tiesinger, J. J., Moritz, S. and Granner, D. K. (1993) J. Biol. Chem. 268,5209-5219 Mayer, D., Klimek, F., Hacker, H. J., SeelmannEggebert, G. and Bannasch, P. (1989) in Liver Cell Carcinoma (Bannasch, P., Keppler, D. and Weber, G., eds.), pp. 329-345, Kluwer Academic Publishers, Dordrecht Mayer, D. and Letsch, I. (1989) Cell Biochem....

In order to establish whether growth of glioma cells is associated with glucose transport and metabolism, we investigated expression of the glucose transporter and hexokinase, as well as glucose transport and glucose phosphorylation in rat C6 glioma cells growing at different rates. Rat C6 glioma cells were subcloned to produce four different cell lines (CL1, CL2, CL3 and CL4) differing in grow...

SUMMARY Concentrations of hexokinase in 18 types of transplanted rat tumors were significantly correlated with the measured growth rates and also paralleled the degrees of loss of histological differentiation of these tumors. The correlations confirm the similar relationship already found among hepatomas and extends it to some nonhepatic tumors of the rat. The normal tissues, fetal and regenera...

To determine hexokinase activity in intact red cells, one may either measure the disappearance of glucose or the appearance of glucose 6-phosphate. The former is not absolutely specific for the hexokinase reaction. The latter can only be done after the cells are broken because phosphorylated compounds are reluctant to move across red cell membranes. Alternatively, studies of hexokinase activity...

To determine hexokinase activity in intact red cells, one may either measure the disappearance of glucose or the appearance of glucose 6-phosphate. The former is not absolutely specific for the hexokinase reaction. The latter can only be done after the cells are broken because phosphorylated compounds are reluctant to move across red cell membranes. Alternatively, studies of hexokinase activity...

Saccharomyces cerevisiae has two homologous hexokinases, I and II; they are 78% identical at the amino acid level. Either enzyme allows yeast cells to ferment fructose. Mutant strains without any hexokinase can still grow on glucose by using a third enzyme, glucokinase. Hexokinase II has been implicated in the control of catabolite repression in yeasts. We constructed null mutations in both hex...

Degradation of Gram-positive bacterial cell wall peptidoglycan in macrophage and dendritic cell phagosomes leads to activation of the NLRP3 inflammasome, a cytosolic complex that regulates processing and secretion of interleukin (IL)-1β and IL-18. While many inflammatory responses to peptidoglycan are mediated by detection of its muramyl dipeptide component in the cytosol by NOD2, we report her...

The dye tetraiodofluorescein (TIF) was found to be an effective inhibitor of yeast hexokinase. It is a competitive inhibitor relative to MgATP2- and a noncompetitive inhibitor of glucose binding, a kinetic pattern consistent with the previously proposed random kinetic mechanism. TIF interacts directly with the native dimeric protein to give a difference spectrum with a maximum at 543 nm. Monome...

Maternal glucose levels during pregnancy impact the developing fetus, affecting metabolic health both early and later on in life. Both genetic and environmental factors influence maternal metabolism, but little is known about the genetic mechanisms that alter glucose metabolism during pregnancy. Here, we report that haplotypes previously associated with gestational hyperglycaemia in the third t...