extraction of intact quality dna from plant tissues, especially those rich in secondary metabolites, is often challenging. literally, hundreds of different dna isolation protocols from various plant species have been published over the last decades. although many commercial dna isolation kits are convenient and designed to be safe, their cost and availability cause limitations in small molecula...

high levels of regulated oncogen-alpha (gro-a) expression have been observed in the liver. gro-a stimulates proliferation of epithelial cells and induction of rolling and extravascular migration of neutrophils and mononuclear cells. given the above observations, this chemokine was chosen to be analyzed in freshly isolated and cultured hepatocytes. in this study, hepatocytes (2×106 cell/ml) were...

Abstract Introduction High-power short-duration (HPSD) radiofrequency ablation has been proposed as a method for producing rapid and effective lesions pulmonary vein isolation (PVI). We aimed to evaluate the procedural characteristics first-pass (FPI) rate of HPSD very high-power (vHPSD) compared low-power long-duration (LPLD) technique. Methods 156 patients with atrial fibrillation (AF) were e...

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Herpes simplex virus types 1 (HSV-1) and 2 (HSV-2) are members of the Herpesviridae family. About 40% to 80% of the world populations are infected with HSV and its prevalence is high in Iran. The high prevalence of this virus in the community and the ability of the virus in causing fatal diseases among immunocompromised patients, have encouraged studies to be performed on HSV and suitable cell ...

Isolation of high-quality RNA is one of the most crucial methods in molecular biology. RNA extraction from woody plants has been problematic due to the presence of rigid and woody tissues, large amounts of polysaccharides, polyphenols and other secondary metabolites. Here we present a suitable protocol for RNA isolation from a wide range of woody plants that includes eight gymnosperms and four ...