an accurate and sensitive reversed-phase high-performance liquid chromatograph-ic method for determination of sertraline in human serum is described using 4-chloro-7-nitrobenzofurazan as pre-column derivatization agent. the drug and an internal standard (azithrimycin) were extracted from serum using a mixture of diethyl ether-chloroform and subjected to the pre-column derivatization with the re...

This article describes a simple and fast high-performance liquid chromatography method for the determination of methotrexate (MTX) in plasma. Samples were collocted from children receiving high-dose MTX at shafa Hospital (Ahvaz University of Medical sciences, Ahvaz, iran) at various times after the end of eachinfusion. Plasma was deproteinized with trichloroacetic acid and the supernatant was i...

This article describes a simple and fast high-performance liquid chromatography method for the determination of methotrexate (MTX) in plasma. Samples were collocted from children receiving high-dose MTX at shafa Hospital (Ahvaz University of Medical sciences, Ahvaz, iran) at various times after the end of eachinfusion. Plasma was deproteinized with trichloroacetic acid and the supernatant was i...

in this study, for the analysis of human urine samples, a novel method explained for the determination of celecoxib, a nonsteroidal anti-inflammatory drug (nsaid), using molecularly imprinted solid-phase extraction (mispe) coupled with high-performance liquid chromatography (hplc). the synthesis of the mip was performed by precipitation polymerization in methacrylic acid (maa), ethylene glycol ...

a solid phase extraction sorbent based on polyaniline/graphene nanocomposite is presented. the structure and morphology of synthesized nanocomposite were investigated by fourier transform infrared spectroscopy, field emission scanning electron microscopy, x-ray diffraction and thermal gravimetric analysis. the dispersive solid phase extraction was employed to the isolation and preconcentration ...

Efficient and robust separation methods are indispensable in modern LC-MS based metabolomics, where high-resolution mass spectrometers are challenged by isomeric and isobaric metabolites. The optimization of chromatographic separation hence remains an invaluable tool in the comprehensive analysis of the chemically diverse intracellular metabolome. While it is widely accepted that a single metho...

objective(s):the present study reports the development and validation of a sensitive and rapid extraction method beside high performance liquid chromatographic method for the determination of crocetin in human serum. materials and methods:the hplc method was carried out by using a c18 reversed-phase column and a mobile phase composed of methanol/water/acetic acid (85:14.5:0.5 v/v/v) at the flow...

Gas chromatography (GC) is generally believed to be restricted to the analysis of ‘volatiles’ and is less applicable to the analysis of so-called ‘heavy’ compounds. The introduction of persilylated glass and fused silicea columns, of thermostable stationary phases and of non-discriminative injection devices in capillary GC (CGC) have made the deRnitions of ‘volatile’ and ‘heavy’ very Sexible. H...

The degradation of irradiated human insulin in aqueous solutions was investigated in order to protect the protein against ionizing radiation. The influence of the drug concentration, excipients and irradiation temperature were studied. Aqueous solutions at pH 2 were irradiated by gamma rays or by accelerated electrons. Two different high-performance liquid chromatography (HPLC) methods were use...

Previous work demonstrates that a flame ionization detector (FID) may be used as a detector for high-temperature water separation. However, the relatively high flow rate of the eluent required by standard high-performance liquid chromatography columns causes instability of the FID. In this work, micro-bore columns are packed with poly(styrene-divinylbenzene) (PRP-1) or Daiso gel octadecylsilane...