The minor satellite DNA of mouse is believed to constitute the centromere. We report that centromeres of some chromosomes in the Cl1D cells of mouse are not associated with this DNA even though the latter is present on these chromosomes. The satellite DNA was detected distally from the centromere and could not be mistaken as a component of the centromere. We also report that the site of the pri...

The organization and replication of intranucleolar DNA in polytene cells of Drosophila nasuta have been examined. Normal Giemsa and fluorescent (Hoechst 33258 and quinacrine mustard) staining reveal that in D. nasuta polytene cells, the intranucleolar DNA is organized into condensed and disperse forms and very often shows connexion to the alpha heterochromatin of the chromocentre. Results of [3...

A female with Duchenne muscular dystrophy, diagnosed at the age of 3 years 8 months, is reported. Chromosome studies revealed an X;autosome reciprocal translocation t(X;5) (p21.2;q31.2). With the BrdU-Hoechst 33258-Giemsa technique, there was nonrandom preferential inactivation of the normal X. Our patient is the ninth reported case of Duchenne muscular dystrophy associated with an X;autosome t...

A fully optimized staining method for detecting sister chromatid exchanges in cultured cells is presented. The method gives reproducibly robust quantitative results. Sister chromatid exchange is a classic toxicology assay for genotoxicity and for detecting alterations to the biochemistry underlying cellular homologous recombination. Growth of cells in the presence of 5'-bromo-deoxyuridine for t...

This study aimed to investigate the anticarcinogenic and genotoxic damage potentials of malic acid on human fibroblast cells (HDFa) glioblastoma (U87-MG) cell lines. MTT viability LDH release assays were performed understand cytotoxic features different Also, Hoechst 33258 fluorescent staining was used monitor nuclear abnormalities including micronucleus, lobbed, notched structures. Furthermore...

T-PM-Pol USE OF THE DYE HOECHST 33258 IN A MODIFICATION OF THE BROMODEOXYURIDINE PHOTOLYSIS TECHNIQUE FOR THE ANALYSIS OF DNA REPAIR. B. S. Rosenstein* and R. B. Setlow (Intr. by M. Simon), Biology Dept., Brookhaven Nation¶l Laboratory, Upton, N.Y. 11973. A modification of the BrdU photolysis technique used in the measurement of the repair of damage in DNA has been developed employing the bisbe...

The crystal structure is reported of a complex between the dodecanucleotide sequence d(CGCGAATTCGCG)2and an analogue of the DNA binding drug Hoechst 33258, in which the piperazine ring has been replaced by an amidinium group and the phenol ring by a phenylamidinium group. The structure has been refined to an R factor of 19.5% at 2.2 A resolution. The drug is held in the minor groove by five str...

Background: Liver cancer is associated with a high mortality rate worldwide. Hepatocellular carcinoma (HCC) constitutes large proportion of primary liver cancers, and most its alterations currently remain untreatable. Diallyl trisulfide (DATS), the main chemical constituent allicin, affects tumour development by regulating cell apoptosis. Allicin-induced autophagy could contribute to apoptosis ...