From 1998 to 2000, 5,267 horse sera were collected from several Trichinella regions in Romania. Sera were initially screened in laboratories in Romania, Serbia and Italy with an ELISA and a Western blot (Wb) using an excretory/secretory (ES) antigen and several conjugates (protein A, protein G, and sheep or goat anti-horse). Differences in serology results were obtained among the different conj...

To provide a convenient and sensitive method for the detection of equine arteritis virus (EAV)-specific serum antibodies, we developed an immunoblot assay employing the EAV nucleocapsid (N) and membrane (M) proteins expressed in a procaryotic expression vector (pMAL-c2) for the production of recombinant maltose-binding (MBP) fusion proteins (MBP-N and MBP-M). The antigenic reactivity of the rec...

In this study, 162 horses, 80 donkeys and 51 mule serum samples were collected in Konya city. Additionally, 64 horse serum samples from Ankara and 49 samples from Kayseri city were included in the study. A total of 406 serum samples were examined by agar gel immunodiffusion (AGID) and enzyme-linked immunosorbent assay (ELISA) for antibody to equine infectious anaemia virus (EIAV) and no positiv...

The purpose of this study was to determine what happens to normal aqueous lysozyme levels during experimentally induced uveitis. Horse-serum-induced uveitis increased the average rabbit aqueous lysozyme value (0.9 mug per milliliter) to 13.8 mug per milliliter, but did not elevate the average serum lysozyme value (4.0 mug per milliliter). These findings suggest that ocular inflammation alone is...

1. Filtrates from B. coli, B. typhosus, or meningococci injected into the skin of guinea pigs do not produce visible inflammation. When these injections are followed by intravascular injections of the same material, hemorrhages do not occur in the skin. 2. Guinea pigs sensitized to horse serum react with redness and edema to 0.1 or 0.01 cc. of horse serum injected into the skin, and subsequent ...

Field and Teague 1 have recently shown that toxins and antitoxins under the influence of the electric current migrate toward the cathode, and in a second series of experiments they found that the agglutinins of typhoid immune serum also travel toward the cathode. With hemolysins the conditions are somewhat more complicated than with toxins or agglutinins owing to the presence of two factors, am...