investigated by immunoblot with serum from individuals sensitized to anisakis or shrimp. IgE binding patterns in the immunoblots were used for the identification of major Anisakis allergens, which were analysed by mass spectrometry-based proteomics in ESI-Orbitrap, after separation on SDS-gel. Results: Four new allergen candidates were identified. The first identified allergen was enolase, whic...

Allergic rhinitis affects up to 40% of children but is commonly undiagnosed. Careful assessment of nasal symptoms allows for the most appropriate therapeutic options to be chosen. Allergen avoidance is often difficult in practice. Antihistamines are of limited benefit in allergic rhinitis caused by house dust mite and other perennial allergens, where symptoms, predominantly nasal obstruction, a...

investigated by immunoblot with serum from individuals sensitized to anisakis or shrimp. IgE binding patterns in the immunoblots were used for the identification of major Anisakis allergens, which were analysed by mass spectrometry-based proteomics in ESI-Orbitrap, after separation on SDS-gel. Results: Four new allergen candidates were identified. The first identified allergen was enolase, whic...

Pillows are known to contain significant levels of indoor allergens and endotoxin, that are of importance to house dust mite sensitized asthmatics. Buckwheat pillows are commonly used in Korea. We studied the levels of the house dust mite allergen, Der f 1, and endotoxin on new synthetic and buckwheat pillows and their accumulation over three months. Endotoxin levels were significantly higher o...

House dust mites produce inhalant allergens of importance to allergic patients. We measured the major group 1 allergens, Der p 1 and Der f 1, from the house dust mites Dermatophagoides pteronyssinus and Dermatophagoides farina, respectively in 100 randomly selected domestic homes from Cheonan, Korea. Dust samples were collected by vacuuming from the living room floor and 1 mattress in each home...