The standardization of Leaves of Mallotus philippensis (Lam.) Mull. Arg. was carried out according to the quality control methods suggested by WHO. The powder material was tested for various parameters like macroscopy, microscopy, foreign matter, ash value, extractable matter, crude fibre content, moisture content, swelling index, foaming index, pesticide residues, Arsenic and heavy metals, mic...

Objective: Boswellic acids (BAs) are active constituents of plant Boswellia serrata. In the present study boswellic acids were extracted from Boswellia serrata and dry methanolic extracts were quantified by HPLC and HPTLC. Method: A gradient HPLC method was used for the quantification of the boswellic acids; 11-keto-β-boswellic acid (KBA) and 3acetyl-11-keto-β-boswellic acid (AKBA). A direct HP...

Hundred Fusarium culmorum strains, isolated from freshly harvested maize grain samples from Southern parts of India, were incubated in czapek-dox medium and analyzed for trichothecene (DON/NIV) production. The mPCR assay was standardized targeting trichothecene metabolic pathway genes viz., Tri6, Tri7, Tri13 for detection of trichothecene (DON/NIV) chemotypes and rDNA gene for specific detectio...

To estimate phenols and flavonoids, enzymic antioxidants in the leaves of Boerhaavia erecta (LBE). The sample was extracted with 0.1M phosphate buffer for the analysis of phenols, flavonoids and antioxidants. The total phenolic content and total flavonoids in LBE were measured using Folin-Ciocalteau and aluminium chloride colorimetric methods respectively. The activity of Superoxide Dismutase (...

Article history: Received on: 05/05/2016 Revised on: 17/07/2016 Accepted on: 19/10/2016 Available online: 31/01/2017 Antimicrobial resistance (AMR) is among the threats to global public health, with developing countries being mostly affected. The AMR has undermined development of new antimicrobial agents by pharmaceutical industries despite new emerging infections. This necessitates a search fo...

Aim of this study was to develop a TLC and a HPTLC fingerprint profiles for various secondary metabolites of methanol extracts of the stem of the traditional medicinal climber, Solena amplexicaulis. These studies were carried out as per the methods of Harborne and Wagner et al. The profiles of various individual secondary metabolites were made and developed for authentication. The methanol extr...

Mucuna pruriens Linn. is an important medicinal plant used for treatment of Parkinson's disease and many others in ancient Indian medical system. L-DOPA extracted from seeds of Mucuna is a constituent of more than 200 indigenous drug formulations and is more effective as drug than the synthetic counterpart. A densitometric high performance thin-layer chromatographic (HPTLC) method was developed...

Alpinetin is a flavonoidal constituent of seeds of Amomum subulatum Roxb., recently reported to possess vasorelaxant and antiHIV activities. Simple, accurate and precise HPLC and HPTLC methods were developed for the analysis of alpinetin in A. subulatum seed extracts and extraction technique was optimized to get maximum yield using conventional, ultrasonic and matrix solid phase dispersion extr...

A simple, rapid, and reliable HPTLC method has been established for simultaneous determination of Nebivolol and S-Amlodipine in pharmaceutical dosage forms. Identification and quantification were performed on silica gel 60 F254 HPTLC plates, prewashed with methanol, with chloroform:toluene:methanol:glacialacetic acid, (5:2:3:0.1, v/v/v/v), as mobile phase. This system furnished compact bands fo...