Hsp70 chaperones assist in a large variety of protein-folding processes in the cell. Crucial for these activities is the regulation of Hsp70 by Hsp40 cochaperones. DnaJ, the bacterial homologue of Hsp40, stimulates ATP hydrolysis by DnaK (Hsp70) and thus mediates capture of substrate protein, but is also known to possess chaperone activity of its own. The first structure of a complete functiona...

Molecular chaperones from Hsp90 and Hsp70 families are known to bind stably to the mutant p53 tumor suppressor protein, and transiently to the wild-type p53 in cells and in vitro [1, 2]. Using highly purified human recombinant proteins, we reconstituted the in vitro chaperone-dependent reactions of p53 binding to the WAF1 promoter-derived sequence. At the physiological temperature of 37°C, Hsp9...

background: hearing loss (hl) is the most frequent neurosensory impairment. hl is highly heterogeneous defect. this disorder affects 1 out of 500 newborns. this study aimed to determine the role of dfnb2 locus and frequency of myo7a gene mutations in a population from west of iran. methods: thirty families investigated in shahrekord university of medical sciences in 2014, genetic linkage analys...

Export of parasite proteins into the host erythrocyte is essential for survival of Plasmodium falciparum during its asexual life cycle. While several studies described key factors within the parasite that are involved in protein export, the mechanisms employed to traffic exported proteins within the host cell are currently unknown. Members of the Hsp70 family of chaperones, together with their ...

found to be a minor component of amyloid plaques in Alzheimer's disease [19]. Alternatively, it is possible that there is an inhibitor of prion generation and propagation whose activity is inhibited by an excess of another prion. Excess Hsp104p cures [PSI + ] [20], excess Ydj1p (an Hsp40) can cure [URE3] [21], and Sis1p (another Hsp40) cures [RNQ + ] [22]. Overexpression of Ssb members of the H...

Diffusion coefficients of huntingtin (Htt) fragments and SOD1 mutants expressed in cells were measured using fluorescence correlation spectroscopy. The diffusion mobilities of both non-pathological Htt fragments (25 polyQs) and pathological Htt fragments (103 polyQs) were much slower than expected for monomers suggesting that they oligomerize. The mobility of these fragments was unaffected by d...

Background: P53, p63, and p73 transcription factors which are belong to The p53 family, are conserved during evolution. They have important roles in many molecular and cellular functions, including tumor suppression, the development of epithelial cell layers, and the development of central nervous system and immune system. Studies show these molecules also have role in maintaining the genomic i...

Heat-shock proteins (HSPs), which are encoded by conserved gene families in plants, crucial for development and responses to diverse stresses. However, the wheat (Triticum aestivum L.) HSPs have not been systematically classified, especially those involved protecting plants from disease. Here, we classified 119 DnaJ (Hsp40) (TaDnaJs; 313 genes) 41 Hsp70 (TaHsp70s; 95 into six four groups, respe...