HuTu 80 and HT 29 are two human gastrointestinal tumor cell lines which differ 20-fold in their sensitivity to growth inhibition by 5-fluorouracil (FUra) [concentration of inhibitor necessary to inhibit cell growth 50% as compared with control cells grown under identical conditions (except that inhibitor was omitted): HuTu 80, 6 microM; HT 29, 0.3 microM]. Drug response in the two cell lines is...

PURPOSE To evaluate the proinflammatory effect and molecular mechanism of IL-17 in the intestinal epithelial cell line HT-29. METHODS After culture of HT-29 cells with IL-17 and/or TNF-(α), real-time (RT) PCR and Western blot were used to measure the gene expression level of the neutrophil chemokines CXCL1, CXCL2, CXCL5, CXCL6, IL-8 and the Th-17 chemokine CCL20, the phosphorylation level of ...

Aim: The aim of our study is to the evaluation melatonin (MLT) and Momordica charantia (MC) combination on PC-3 HT-29 cancer lines address question where or not MLT increases MC antitumor effect in lines. 
 Material Method: cell were grown a manufacturer-specified culture medium. Cisplatin, MLT, increasing concentrations MC, 40 ?g/ml + applied for 72 hours. 3-(4,5-Dimethylthiazol-2-Yl)-2,5...