Aim The aim of this study was to examine the potential ameliorative effects caffeic acid (CA) on hydrogen peroxide (H2O2)-induced neurodegeneration in a human SH-SY5Y cell line, as well possible mechanisms involved. Method Cell proliferation evaluated by WST-1 assay. apoptotic index calculated TUNEL Assay. Antioxidant parameters were studied measuring reactive oxygen species (ROS), lipid peroxi...

background noise pollution causes of apoptosis in germinal cells of testes. this study investigated the effects of honey and vitamin e administration on the process of apoptosis in cells of the germinal testis of rats exposed to noise stress. material and methods in this study, 24 male wistar rats randomly grouped as: 1 (honey + noise stress), 2 (vitamin e + noise stress) 3 (noise stress) and g...

Purpose: To investigate the therapeutic role of diosmetin in periodontitis and its probable mechanism action.Methods: Lipopolysaccharide (LPS) was used to induce periodontal cells. Cell viability apoptosis response LPS were evaluated using MTT TUNEL assays. The oxidative stress inflammatory responses LPS-induced effects cells detected enzyme-linked immunosorbent assay (ELISA). In addition, role...

objective(s) cigarette and nicotine enhances embryogenesis, fertility, pregnancy loss and ultrastructure alterations of oocyte. this study was performed to determine the effect of daily supplementation of vitamin e on oocytes apoptosis in nicotine-treated mice. materials and methods in this experimental study, 24 nmari adult female mice were randomly allocated into four experimental groups. for...

Although pyrimethamine (Tindurin™) appears to be effective in the prevention and treatment of some infectious diseases, very little information exists on its unpredictable properties. We design this study to evaluate its anti-tumoral effect on a model of cell line. Methods: The cytotoxic influence of Pyrimethamine on prostate cell line was investigated using an in vitro colometric assay. The po...

Butyrate, a fermentation product of the large bowel microflora, is potentially protective against the development of colorectal cancer. In vitro, butyrate has been shown to induce apoptosis and inhibit proliferation in numerous cancer cell lines, including colorectal cancer. Although these tumor suppressing properties of butyrate are well-documented in experimental systems, the mechanisms under...

These studies report on the activation and induction of cGMP-dependent protein kinase (PKG) by exisulind and analogs and test the hypothesis that PKG is involved in the induction of apoptosis in colon tumor cells. Exisulind and analogs are proapoptotic drugs developed as inhibitors of cGMP phosphodiesterase gene families 5 and 2 that have been shown to sustain increased cGMP in SW480 and HT29 c...

Abstract Background Long non-coding RNA is considered to be essential modulate the development and progression of human malignant cancers. And long can act as crucial modulators by sponging corresponding microRNA in tumorigenesis. We aimed elucidate function ACTA2-AS1 its molecular mechanism colon adenocarcinoma. Materials methods The expression ACTA2-AS1, miR-4428 BCL2L11 adenocarcinoma tissue...

The electrophoresis pattern of apoptotic cells detected by the comet assay has a characteristic small head and spread tail. This image has been referred to as an apoptotic comet, but it has not been previously proven to be apoptotic cells by any direct method. In order to identify this image obtained by the comet assay as corresponding to an apoptotic cell, the frequency of appearance of apopto...

BACKGROUND Volatile anesthetics attenuate apoptosis. The underlying mechanisms remain undefined. The authors tested whether isoflurane reduces apoptosis in cardiomyocytes subjected to oxidative or inflammatory stress by enhancing Akt and B-cell lymphoma-2 (Bcl-2). METHODS Adult and neonatal rat ventricular myocytes and atrial HL-1 myocytes were exposed to hypoxia, hydrogen peroxide, or neutro...