Hyperosmolar therapy is the principal medical management strategy for elevated intracranial pressure. Mannitol has been the primary hyperosmolar agent for nearly a century and remains the de facto gold standard for medical management of intracranial hypertension. Over the past 25 years, however, hypertonic saline (HTS) has become a progressively more common alternative to mannitol, and several ...

Adducin is a ubiquitously expressed actin- and spectrin-binding protein involved in cytoskeleton organization, and is regulated through phosphorylation of the myristoylated alanine-rich C-terminal kinase (MARCKS)-homology domain by protein kinase C (PKC). We have previously shown that the Drosophila adducin, Hu-li tai shao (Hts), plays a role in larval neuromuscular junction (NMJ) growth. Here,...

The application of genome scale RNA interference (RNAi) relies on the development of high quality RNAi high throughput screening (HTS) assays. An important quality control (QC) characteristic in an HTS assay is how well the positive controls, samples, and negative controls can be separated from each other in the assay. Signal-to-noise ratio, signal-to-background ratio, Z-factor and Z’-factor ha...

PURPOSE Trauma-induced suppression of cellular immune function likely contributes to sepsis, multiple organ dysfunction syndrome and death. T cell proliferation decreases after traumatic stress. The addition of prostaglandin E(2) (PGE(2)), which depresses immune function after hemorrhage and trauma, to T-cells decreases T-cell proliferation; and hypertonic saline restores PGE(2)-induced T-cell ...

We analyzed the structure of cytoplasmic bridges called ring canals in Drosophila egg chambers. Two mutations, hu-li tai shao (hts) and kelch, disrupt normal ring canal development. We raised antibodies against the carboxy-terminal tail of hts and found that they recognize a protein that localizes specifically to ring canals very early in ring canal assembly. Accumulation of filamentous actin o...

Development of RNA and DNA aptamers for diagnostic and therapeutic applications is a rapidly growing field. Aptamers are identified through iterative rounds of selection in a process termed SELEX (Systematic Evolution of Ligands by EXponential enrichment). High-throughput sequencing (HTS) revolutionized the modern SELEX process by identifying millions of aptamer sequences across multiple rounds...

In this paper the preliminary results, of a new approach on speech modeling for statistical parametric HMM-based speech synthesis are presented. The proposed system is based on a flexible pitch-asynchronous harmonic/stochastic model (HSM) [1]. The speech is modeled as the superposition of two components: a harmonic component and a stochastic or aperiodic component. The fact that the synthesis m...

High-throughput screening (HTS) of chemical compounds to identify modulators of molecular targets is a mainstay of pharmaceutical development. Increasingly, HTS is being used to identify chemical probes of gene, pathway, and cell functions, with the ultimate goal of comprehensively delineating relationships between chemical structures and biological activities. Achieving this goal will require ...

It has been known for some time that high-temperature superconductors (HTS) are critical for the construction of NMR magnets generating 1 GHz and above. Such systems generally require an HTS insert to be placed in the inner high-field region of a low temperature superconducting (LTS) magnet. Among the problems in the presently available HTS technology are splice resistance and index dissipation...

Background: Acute bronchiolitis is a common lower respiratory tract infection in infants which results from inflammatory obstruction of the small airways, Respiratory Syncytial Virus (RSV) being the main etiological agent. Aim and Objectives: To compare the effectiveness of 3% HTS nebulization with 0.9% NS nebulization , In hospitalized children with acute bronchiolitis based on time for resolu...