نتایج جستجو برای: human ovarian tissue culture

تعداد نتایج: 2579051  

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1999
N Auersperg J Pan B D Grove T Peterson J Fisher S Maines-Bandiera A Somasiri C D Roskelley

Ovarian carcinomas are thought to arise in the ovarian surface epithelium (OSE). Although this tissue forms a simple epithelial covering on the ovarian surface, OSE cells exhibit some mesenchymal characteristics and contain little or no E-cadherin. However, E-cadherin is present in metaplastic OSE cells that resemble the more complex epithelia of the oviduct, endometrium and endocervix, and in ...

2011
Michael J Bradaric Animesh Barua Krishna Penumatsa Yu Yi Seby L Edassery Sameer Sharma Jacques S Abramowicz Janice M Bahr Judith L Luborsky

BACKGROUND Sphingosine-1 receptor 1 (S1P1) plays a major role in regulating lymphocyte egress from peripheral lymph tissue. Lymphocyte trafficking is potentially a critical response to tumors and to tumor vaccines. Also, the receptor has been shown to influence metastasis. However, there is little information on its expression in the aged ovary or ovarian tumors. As a basis for further studies ...

2017
Mahboobeh Amoushahi Mojdeh Salehnia Seyed Javad Mowla Nassim Ghorbanmehr

OBJECTIVE This study aimed to evaluate the expression of the genes related to folliculogenesis after vitrification of mouse ovarian tissues using a two-step in vitro culture. MATERIALS AND METHODS In this experimental study, vitrified and non-vitrified ovaries from 7- day old (neonate) female mice were cultured using alpha-Minimum Essential Medium (α-MEM) supplemented with 5% fetal bovine ser...

2017
Shuo Xiao Jonathan R Coppeta Hunter B Rogers Brett C Isenberg Jie Zhu Susan A Olalekan Kelly E McKinnon Danijela Dokic Alexandra S Rashedi Daniel J Haisenleder Saurabh S Malpani Chanel A Arnold-Murray Kuanwei Chen Mingyang Jiang Lu Bai Catherine T Nguyen Jiyang Zhang Monica M Laronda Thomas J Hope Kruti P Maniar Mary Ellen Pavone Michael J Avram Elizabeth C Sefton Spiro Getsios Joanna E Burdette J Julie Kim Jeffrey T Borenstein Teresa K Woodruff

The endocrine system dynamically controls tissue differentiation and homeostasis, but has not been studied using dynamic tissue culture paradigms. Here we show that a microfluidic system supports murine ovarian follicles to produce the human 28-day menstrual cycle hormone profile, which controls human female reproductive tract and peripheral tissue dynamics in single, dual and multiple unit mic...

2017
Xiao Wan Phurit Bovornchutichai Zhanfeng Cui Eric O'Neill Hua Ye

Antiangiogenic therapy for cancer is a strategy targeted at tumour vasculature, often in combination with conventional cytotoxicity treatments. Animal testing is still the most common method used for evaluating the efficacy of new drugs but tissue-engineered in vitro models are becoming more acceptable for replacing and reducing the use of animals in anti-cancer drug screening. In this study, a...

Journal: :Zygote 2016
M A Filatov Y V Khramova M V Kiseleva I V Malinova E V Komarova M L Semenova

In the present review, the main strategies of female fertility preservation are covered. Procedures of fertility preservation are necessary for women who suffer from diseases whose treatment requires the use of aggressive therapies, such as chemotherapy and radiotherapy. These kinds of therapy negatively influence the health of gametes and their progenitors. The most commonly used method of fem...

Journal: :caspian journal of enviromental sciences 0

the aim of the present study was to develop an in vitro system for functional investigation of ovarian follicular cells in sterlet, acipenser ruthenus. oocytes for the primary culture were obtained from the ovaries of a 6 years old sterlet 729 g in weight and 47 cm in total length. the oocytes were in advanced vitellogenesis stage (pi >10). a part of the ovary (containing about 300 follicles) w...

B Ebrahimi NS Abtahi R Fatehi, SZ Sadr

Background This research was conducted to assess preantral follicle development in fibrin alginate matrix following ovarian tissue vitrification MaterialsAndMethods Ovaries of 13-day-old NMRI female mice were removed and placed in control and vitrification groups. Vitrification group ovaries were transferred in media containing ethylene glycol, dimethyl sulphoxide, and sucrose then were plunged...

2013
Huiyu Zhuang Jian Gao Zhenhua Hu Juanjuan Liu Dawo Liu Bei Lin

OBJECTIVE The main aims of this study were to explore the molecular structural relationship between Human epididymis protein 4 (HE4) and Lewis y antigen by determining their expression patterns and clinical significance in ovarian epithelial carcinoma. METHODS The structural relationship between HE4 and Lewis y antigen was examined using immunoprecipitation and confocal laser scanning microsc...

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