A rapid method for nearly quantitative isolation of human serum albumin is described. The present technic (1) is a modification of the Schwert method (2). In the present method 4 ml. of serum is precipitated with trichloroacetic acid in a final concentration of 5% (w/v). The precipitate is washed with TCA, and after aqueous suspension, ethanol is added to a final concentration of 80%. The undis...

The binding of bilirubin was analysed in the serum of a patient with unusual myeloma. This patient presented an acquired Von Willebrand's disease due to the presence of a ternary complex formed by one molecule of myeloma immunoglobulin G and two molecules of albumin (Harlé et al. La Presse Médicale 19, 1661-1664 (1990)). The binding characteristics of bilirubin--number of sites (n) and associat...

It has been suggested by Wiener and Hurst (1947) and Wiener, Hurst, and Sonn-Gordon (1947) that mixtures of bovine albumin and human plasma are more effective as diluents for the detection of incomplete antibodies than the widely employed 20% bovine albumin introduced by Diamond and Abelson (1945) or the human plasma alone used by Wiener (1945). This has been confirmed by other workers, and Mol...