1. The conditions required for sensitive and specific colorimetric determination of proline with acidified ninhydrin were investigated. 2. A method applicable to protein samples was developed. 3. The only compound found to interfere appreciably was a hydroxyproline.

Background & Objectives: Hepatic fibrosis is the extreme extracellular matrix buildup of collagen due to chronic liver disorders. Progressive results in cirrhosis and hepatic failure. Nilotinib considered a 2nd generation tyrosine kinase inhibitor, which exhibited antifibrotic efficacy. Stem cells release cytokines growth factors paracrine-mediated anti-inflammatory effects vitro vivo. Hence, s...

The total nitrogen and L-hydroxyproline concentrations of two intra-abdominal and two subcutaneous sites of adipose tissue were measured in male and female Wistar rats. At 100 g body weight the nitrogen concentration of intra-abdominal adipose tissue was slightly lower than that of subcutaneous adipose tissue. As age advanced beyond sexual maturity, the nitrogen content of intra-abdominal adipo...

Incubation of soybean hypocotyl sections with 0.1 millimolar 2,2'-dipyridyl in the absence of auxin results in increases in growth rate and in cell wall extensibility lasting for about 3 hours. This is accompanied by greatly decreased biosynthesis of hydroxyproline, which ultimately appears in the wall, and in slightly reduced oxygen uptake, both of which continue for at least 9 hours. Continuo...

Selected parameters of quality (hydroxyproline, calcium, bone particles content, and histological determination of bone particles) of mechanically deboned poultry meat (MDPM -„firm separation“ and soft separation „Baader meat“) were compared. Comparison of results with composition (except bone particles) of fresh poultry meat (breast and thigh muscles) was performed. Hydroxyproline content was ...

A high-performance hquid chromatographic assay for total hydroxyproline in serum and gingival crevicular fiuid has been developed. The Clq component of the first complement factor was precipitated out of the samples with a 0.02 M sodium acetate solution. Ahquots of the supernatants were dried, vapor hydrolyzed with 6 M hydrochloric acid at 105°C for 24 hours and derivatized with phenylisothiocy...