105 Keyword Index 106 Introduction 106 Results/Discussion 108 Conclusions and Concluding Remarks 117 Experimental 117 Acknowledgements 122 Literature Cited 123 Tables 127 Figure Legends 131 Figures 133 CHAPTER 5. IDENTIFICATION OF PATHWAYS IMPORTANT FOR THE ANTI-INFLAMMATORY ACTIVITY OF AN HYPERICUM PERFORATUM FRACTION AND FOUR PUTATIVE BIOACTIVE CONSTITUENTS IN RAW 264.7 MOUSE MACROPHAGES USIN...

The essential oil isolated from fresh aerial parts of Hypericum perforatum L. was analyzed by GC and GC/MS. One hundred and thirty four identified compounds accounted for 98.7% of the total oil. The main components of the oil were: germacrene D (18.6%), (E)-caryophyllene (11.2%), 2-methyloctane (9.5%), α-pinene (6.5%), bicyclogermacrene (5.0%) and (E)-β-ocimene (4.6%). The volatile profile of H...

OBJECTIVES Hypericum perforatum L. is a medicinal plant containing many polyphenolic compounds, ‎including flavonoids and phenolic acids with antidepressant and anti-inflammatory properties. ‎This study was investigated the effects of Hypericum perforatum extract (HPE) on immunity, ‎body weight (BW), and spleen index (SI) in rats.‎ MATERIALS AND METHODS A total of 24 Wistar male rats were ran...

Oliver St John Gogarty (1878-1957) was a celebrated Irish doctor and poet. A 'Renaissance' figure, he was renowned as a surgeon, poet, memoirist, senator, athlete and wit. His years as a medical student, although not crowned by academic success, were remarkable for many other achievements. A study of Gogarty's University career provides a number of lessons in the art of being a medical student....

In the genus Hypericum, cryoconservation offers a strategy for maintenance of remarkable biodiversity, emerging from large inter- and intra-specific variability in morphological and phytochemical characteristics. Long-term cryostorage thus represents a proper tool for preservation of genetic resources of endangered and threatened Hypericum species or new somaclonal variants with unique properti...

The process extraction of hypericin from the aerial parts Hypericum scabrum and perforatum was studied. On basis results research, following conditions were established: extractant – 80% ethanol, particle size raw material no more than 8 mm temperature 20–40 °C. By method mathematical planning experiment according to Box-Wilson, determined, which ensure with a yield 95%. Proposed five-fold H. n...

The study shows the influence of the origin of plant material and biological source on the in vitro antioxidant (neutralization of DPPH and OH radical, nitric oxide, and inhibition of lipid peroxidation) and anticholinesterase activity of chemically characterized and quantified ethanol extracts of ten St. John's wort samples. The investigated samples were: five Hypericum perforatum species repr...

Hypericum perforatum is a medicinal plant which has been known in traditional medicine as an antiinflammatory and healing agent. Nowadays the use of Hypericum extracts is concerned mainly with antidepressive applications. In the present work, HPLC – RPC18 column chromatography with photodiode array detection was applied for the determination of the derivatives of cinnamic and benzoic acid (e.g....