Abstract The immunological complexity of inflammatory bowel disease (IBD) results in persistent intestinal inflammation millions patients. As an epithelial cell-derived cytokine essential the differentiation CD4+ T helper cells, interleukin 18 (IL-18) is emerging as a therapeutic target for IBD. When naïve cells are stimulated with IL-18, production IFNγ and Th1 increase. IL18R1−/− produce less...

Abstract The central nervous system (CNS) presents a unique immunological environment with restricted infiltration of immune cells from the periphery. Since much is unknown about CNS-infiltrating during infection, we utilized attenuated Venezuelan equine encephalitis virus vaccine strain TC83 (VEEV-TC83) to study T cell responses this viral infection. VEEV-TC83 intracranial (IC) infection wild-...

Phthalates are quasi-ubiquitous plasticizers found in products including building materials, food packaging, clothes, and personal care products. Thus, we permanently exposed to them. endocrine disruptors that can be ingested or absorbed through the skin. Because DEHP is most common phthalate, sought investigate its effect on epidermis. We generated human epidermal equivalents (HEEs) with kerat...

Endometriosis (EM) is a common multifactorial gynaecological disorder. Although Genome-Wide Association Studies have largely been employed, the current knowledge of genetic mechanisms underlying EM far from complete, and other approaches are needed. To this purpose, whole-exome sequencing (WES) was performed on deeply characterised cohort 80 patients aimed at identification rare damaging varian...

Acne and rosacea are both highly prevalent inflammatory skin diseases that characterized by erythema, papules, pustules, primarily involving the pilosebaceous units of face, yet with distinct mechanisms disease pathogenesis. Here, we used single-cell RNA-sequencing (scRNA-seq, n=22) spatial imaging (CosMx, n=4) to compare gene expression individual cells in acne vs. lesions elucidate pathways p...

Abstract BACKGROUND Leptomeningeal disease (LMD) occurs in approximately 5% of patients with breast cancer and has a median survival 2-4 months. We found loss the anti-HER2 anti-HER3 CD4 Th1 immune responses patients. In pre-clinical clinical trials, administration class II HER2 peptide-pulsed dendritic cell vaccine (HER2-DCV) partially restores pathologic complete HER2+ Here, we examined intra...

Idiopathic achalasia is characterized by the absence of peristalsis secondary to loss of neurons in the myenteric plexus that hampers proper relaxation of the lower esophageal sphincter. Achalasia can be considered a multifactorial disorder as it occurs in related individuals and is associated with HLA class II genes, thereby suggesting genetic influence. We used microarray technology and advan...

OBJECTIVE To investigate the differential gene expression of cytokines and compare their impacts on the immune functions among the acute myocardial infarction patients (AMI), the stable angina patients (SA) and the controls. METHODS 20 patients with AMI, 20 patients with SA and 20 healthy volunteers were recruited into the study. Whole human genome microarray analysis was used to detect the g...