Sheep antisera specific for the three major immunoglobulin groups of the rabbit, i.e. IgG (gamma-chain), IgA (alpha-chain), and IgM (micro-chain), are each able to induce blast transformation of the peripheral lymphocytes of the rabbit when added to in vitro cultures. The per cent of lymphocytes transformed with each antiserum indicate that one-fourth of the peripheral lymphocytes carry or have...

in helping pokeweed mitogen-induced immunoglobulin production by normal B cells. However, when normal T cells were co-cultured with B cells from patients with Hodgkin's disease, spontaneous IgG synthesis declined, whereas the addition of patient T cells Received for publication 21 May 1982 and in revisedform 26 November 1982. to normal B cells resulted in an increase of spontaneous IgG synthesi...

Methods for preparing bentonite-gamma globulin complexes and for determining their attachment to cells in tissue culture were investigated by use of human immunoglobulin G (IgG) labeled with 125I. For virus-inhibition studies, bentonite-IgG complexes were prepared by use of human IgG with high specific neutralizing activity against coxsackievirus A21. Much of the antibody in the complex remaine...

Normal human and rabbit sera when incubated with Staphylococcus aureus inhibit the adsorption of bacteriophages. The bacteriophage adsorption was also inhibited by separated normal immunoglobulin M (IgM), F(ab')(2), and Fab-fragments of IgG. No inhibition was obtained with myeloma IgG or Fc-fragments of normal human and rabbit IgG. The results indicate that the serum inhibition of bacteriophage...

An in-house-developed enzyme-linked immunosorbent assay detected West Nile virus (WNV) immunoglobulin A (IgA) in 65 of 68 sera from WNV-infected patients; 40 of 63 WNV IgM-positive, IgG-negative serum or plasma specimens; 65 of 67 WNV IgM-positive, IgG-positive specimens; 0 of 70 WNV IgM-negative, IgG-negative specimens; and 0 of 64 archived blood donation sera. WNV IgA is thus highly prevalent...

In vitro immunoglobulin synthesis by isolated intestinal mononuclear cells of macroscopically normal mucosa from patients with Crohn's disease has been studied and results compared with those obtained with cells from normal mucosa of resection specimens from patients with colonic carcinoma, or other intestinal disorders. The total lamina propria mononuclear cells (LPMC) in Crohn's disease produ...

By immunoelectrophoresis at least three immunoglobulins, IgG, IgA and IgM can be recognized but quantitative determination of each immunoglobulin has been hampered by methodological difficulties. Recently, however, Fahey and McKelvey described in details an antibody-agar plate method for this purpose. While the author was following their method, it way found that less antiserum was required by ...

The purpose of this investigation was to study the distribution and levels of the following immunoglobulins, IgA, IgG, and IgM ,in sera and in some secretions of monkeys (M. fascicularis). IgG, IgA, and IgM were isolated from monkey serum and secretory IgA was separated from monkey milk by combined gel filtration and ion-exchange chromatography. These pure preparations served as standards to qu...

Peripheral blood lymphocytes from 27 healthy individuals and from 18 patients with a diverse spectrum of defects in humoral immunity were examined for their capacity to undergo terminal differentiation in vitro. Pokeweed mitogen induced cells from normal persons to synthesize and secrete IgM. IgG, and IgA as detected by Immunofluorescence and incorporation of [(14)C]amino acids, Lymphocytes fro...

Nineteen canine lymphomas were included in this study. Tumors were classified according to the updated Kiel classification adapted for canine lymphomas by Fournel-Fleury et al. Immunoglobulin light chains (kappa and lambda) and IgM and IgG expression were determined by immunohistochemical method. In all examined cases neoplastic cells were positive for one of the immunoglobulin light chains. Ex...