BACKGROUND Molecular detection methods for HER2/neu gene amplification include fluorescence in situ hybridization (FISH) and competitive PCR. We designed a quantitative PCR system utilizing fluorescent hybridization probes and a competitor that differed from the HER2/neu sequence by a single base change. METHODS Increasing twofold concentrations of competitor were coamplified with DNA from ce...

Detection of RNA viruses by reverse transcription (RT)-PCR has proven to be a useful approach for the diagnosis of infections caused by many viral pathogens. However, adequate controls are required for each step of the RT-PCR protocol to ensure the accuracies of diagnostic test results. Heterologous competitor RNA can be used as a control for a number of different aspects of diagnostic RT-PCR. ...

Two experiments used the head-mounted eye-tracking methodology to examine the time course of lexical activation in the face of a non-phonemic cue, talker variation. We found that lexical competition was attenuated by consistent talker differences between words that would otherwise be lexical competitors. In Experiment 1, some English cohort word-pairs were consistently spoken by a single talker...

This paper investigates the behaviour of Indian aggregate imports during the period 1971-1995. In our empirical analysis of the aggregate import demand function for India, cointegration and error correction modelling approaches have been used. In the aggregate import demand function for India, import volume is found to be cointegrated with relative import price and real GDP. Our econometric est...

Abstract China is a leader in the global transportation industry, with an especially significant position ocean ports. A mapping of every port outside reveals that Chinese firms own or operate terminal assets ninety-six ports fifty-three countries. An original dataset firms' overseas holdings documents geographic distribution, ownership, and operational characteristics these What are internatio...

Predation and competition are critical processes influencing the ecology of organisms, and can play an integral role in shaping coral reef fish communities. This study compared the relative and interacting effects of competition and predation on two competing species of coral reef fish, Pomacentrus amboinensis and P. moluccensis (Pomacentridae), using a multifactorial experiment. Fish were subj...

abstract this study uses gravity model to examine the most important determinants of was agricultural trade. to this aim, import and export statistics of iran agricultural products for the period 1380 to 1387 was provided from iran customs organization and other information was obtained from different internet bases. random effects estimating and hausman test ratio result in fixed effects model...

Current projects in Systems Biology often produce a multitude of different high-throughput data sets that need to be managed, processed, and analyzed in an integrated fashion. In this paper, we present the OmixAnalyzer, a web-based tool for management and analysis of heterogeneous omics data sets. It currently supports gene microarrays, miRNAs, and exon-arrays; support for mass spectrometry-bas...

The fragmentation of production has resulted in an increasing degree of vertical specialization across countries. This paper studies one venue that has facilitated growth in U.S. vertical specialization, the program known as 9802 in the current tariff code, or alternatively as the overseas assembly provision, or OAP, in earlier years. The empirical analysis examines how the cross-country patter...

Amiloride, a diuretic drug that acts by inhibition of various sodium transporters, is toxic to the fission yeast Schizosaccharomyces pombe. Previous work has established that amiloride sensitivity is caused by expression of car1+, which encodes a protein with similarity to plasma membrane drug/proton antiporters from the multidrug resistance family. Here we isolated car1+ by complementation of ...