Customizable endonucleases are providing an effective tool for genome engineering. The resulting primary transgenic individuals (T0) are typically heterozygous and/or chimeric with respect to any mutations induced. To generate genetically fixed mutants, they are conventionally allowed to self-pollinate, a procedure which segregates individuals into mutant heterozygotes/homozygotes and wild type...

background: withania somnifera l. dunal, (ashwagandha) belongs to the family solanaceae and is known as ‘indian ginseng’ in the traditional system of medicine. objectives: in the present investigation, comparative analysis was carried out between two different ecotypes of w. somnifera related to total soluble protein and antioxidant enzyme activity [catalase (cat), superoxide dismutase (sod) an...

abstract during a survey for detection of pvs in potato fields in northern khorasan province, some tubers from the plants which have been already marked and showing mosaic, rugosity, undulation on leaf margins, little leaf and slightly vein deepening, were collected in bodjunrd region, also, some tubers from potato fields of mashhad, chenaran, ghoochan, faruj and shirvan regions were randomly c...

The results of the attempts here described to cultivate the incitant of mosaic disease of tobacco and tomato plants give good reason to suppose that this has been accomplished. The agent in the tissues of affected plants induces the disease not infrequently in a dilution of 1:10,000 but only very rarely at a higher dilution. During its transference from tube to tube of the special medium used i...