In vitro multiple shoot regeneration of Solanum nigrum L., an Indian medicinal plant was accomplished on MS medium utilizing shoot tip and nodal explants. Direct multiple shoots differentiated within 6 weeks when explants were cultured on MS medium containing BAP (1.0-5.0 mg/l) and KIN (1.0-5.0 mg/l) individually. Among various concentrations of cytokinins tested, maximum number of multiple sho...

A complete protocol is presented for in vitro regeneration of pomegranate (Punica granatum L.), a tropical fruit tree, using cotyledonary nodes derived from axenic seedlings. Shoot development was induced from cotyledonary nodes on Murashige and Skoog (1962) (MS) medium supplemented with 2.3±23.0 mM benzyladenine (BA) or kinetin (Kn). Both type and concentration of cytokinin signi®cantly in ̄uen...

in this work, we report the effect of biopolymers (starch and gelatin) on in vitro biocompatibility property of nano hydroxyapatite (nhap) composites. cell culture and mtt assays were performed for in vitro biocompatibility. they show that nhap can affect the proliferation of cells and the nhap-starch and nhap-gelatin biocomposites have no negative effect on the cell morphology, viability and p...

An in vitro micropropagation protocol was developed for Capsicum chinense Jacq. popularly known as habenaro pepper which is one of the world’s hottest chilli and it is a traditional food item of the Naga people. Nagaland is believed to be the original home for the chilli pepper. The Guinness book of World record has awarded this chilli as hottest one as it contains 1,041,427 Scoville Heat units...

The resurrection plant Selaginella pulvinata (Hook. & Grev.) Maxim is used as an ornamental and medicinal plant. It also a good candidate for exploring the desiccation tolerance of plants. However, there not efficient propagation method S. . In present study, we evaluated establishment in vitro using frond tips explants. original shoot induction, adventitious proliferation plantlet growth m...

In vitro propagation of plant has played a very important role in rapidmultiplication of cultivars with desirable traits and production of healthy and disease-free plants. In the present investigation, the objectives were to optimize the micropropagation of Rose hybrid ‘Black Baccarat’ cultivar. In proliferation step, the nodal segments (1.5 cm) was cultured on both liquid and solid media (MS, ...

Atriplex taltalensis is an endangered shrub endemic to northern Chile. Sparse populations of this species can be found in areas with extreme edaphoclimatic conditions the Atacama desert, and its seeds have a poor germination rate. Consequently, mass-cultivating it challenging. In study, vegetative propagation mechanisms A. were evaluated using vitro culture aid their conservation. shoot explant...