Regulation of translation initiation plays a critical role in the control of cell growth and division in eukaryotic cells. Translation of many growth regulatory proteins including cyclins depends critically on translation initiation factors because their mRNAs are translated inefficiently. We report that clotrimazole, a potent antiproliferative agent both in vitro and in vivo, inhibits cell gro...

Nonsense-mediated mRNA decay (NMD) is a surveillance system that eliminates transcripts with premature termination codons. In this study, we show that mRNAs targeted by NMD are also suppressed at the translational level. The low translational efficiency (TE) is a consequence of multiple features acting in concert, including low translation initiation rate, mediated by 5' secondary structure and...

generally, the evaluation and assessment critical condition of rill formation are useful for a better understanding of soil erosion processes. the inherence characteristics of soils, which have much dynamic variations on the hill-slopes and are affected by rill formation, are the soil critical shear stress and soil erodibility factors. this study aims to assess experimental rill incision thresh...

Understanding the pathway and kinetic mechanisms of transcription initiation is essential for quantitative understanding of gene regulation, but initiation is a multistep process, the features of which can be obscured in bulk analysis. We used a multiwavelength single-molecule fluorescence colocalization approach, CoSMoS, to define the initiation pathway at an activator-dependent bacterial σ(54...

Internal ribosome entry sites (IRESs) are important RNA-based translation initiation signals, critical for infection by many pathogenic viruses. The hepatitis C virus (HCV) IRES is the prototype for the type 3 IRESs and is also invaluable for exploring principles of eukaryotic translation initiation, in general. Current mechanistic models for the type 3 IRESs are useful but they also present pa...

Breast tumors contain tumorigenic cancer cells, termed "tumor-initiating cells" (TICs), which are capable of both replenishing themselves and giving rise to populations of nontumorigenic breast cancer cells (non-TICs). However, the molecular mechanisms responsible for breast tumor initiation remain poorly understood. Here we describe a chemical screening strategy to identify small molecules tha...

We propose a new approach called as a peak time approach for faster detection of step initiation for the lower limb exoskeleton. As faster detection of step initiation is an important criterion in evaluating the lower limb exoskeleton, many studies have investigated approaches to detect step initiation faster, including using electromyography, the center of pressure, the heel-off time and the t...

While the main drivers of melanoma initiation, including NRAS/BRAF activation and loss PTEN or CDKN2A, have been identified, role key transcription factors that impose altered transcriptional states in response to deregulated signaling is not well understood. The POU3F2/BRN2 domain factor a regulator invasion, but its initiation remains unknown. Here, BrafV600E PtenF/+ context, we show BRN2 hap...

PURPOSE OF REVIEW In general, the initiation of combination antiretroviral (cART) is associated with improvement in cognitive function. However, the impact of cART has on cognitive function in neurologically asymptomatic HIV-infected individuals initiating therapy at high CD4 lymphocyte cell counts is unknown. RECENT FINDINGS Cognitive function impairment remains prevalent despite effective c...