The presence of indole-3-butyric acid (IBA) as an endogenous auxin in Arabidopsis has been recently demonstrated. However, the in vivo role of IBA remains to be elucidated. We present the characterization of a semi-dominant mutant that is affected in its response to IBA, but shows a wild-type response to indole-3-acetic acid (IAA), the predominant and most studied form of auxin. We have named t...

Indole-3-butyric acid (IBA) was recently identified by GC/MS analysis as an endogenous constituent of various plants . Plant tissues contained 9 ng g fresh weight of free IBA and 37 ng g-' fresh weight of total IBA, compared to 26 ng g-' and 52 ng g-' fresh weight of free and total indole-3-acetic acid (IAA), respectively . IBA level was found to increase during plant development, but never rea...

The present study was conducted in Bangalore. It aims at studying the effect of different concentrations indole-3-butyric acid (IBA) (1000, 2000 and 3000 ppm) on V-1 mulberry cuttings with varying number buds (one, two, three four) consist twelve treatments which were replicated thrice Factorial CRD. results interaction various concentration IBA recorded best budded treated ppm regarding sprout...

Ildole 3 butyric acid (IBA) at 8rng l i induced, vigorous rooting in Bupleurum falcatum L. (Saiko) root culture in B5 medium after about 2weeks, when it was added at the time when detached roots were transferred to fresh medium. The rooting activity markedly decreased with delay of IBA addition after root 2transplantation, and was hardly observed when IBA was added at the 7th day. Stresses such...

Root induction by auxins is still not well understood at the molecular level. In this study a system has been devised which distinguishes between the two active auxins indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA). IBA, but not IAA, efficiently induced adventitious rooting in Arabidopsis stem segments at a concentration of 10 microM. In wild-type plants, roots formed exclusively ou...

Somatic embryos and rooted plantlets have been regenerated from light-initiated embryogenic callus derived from mature embryos of Picea abies stored seeds. Under a 16 hour photoperiod, mature zygotic embryos were cultured on a modified half-strength Murashige-Skoog medium without NH4NO3 and supplemented with 5 mM glutamine, 4.5 pM N6-benzyladenine (BA) and 10.7 PM naphthalene-acetic acid (NAA) ...

The sequence of histogenesis and organogenesis in the calus of Hyssopus officinalis nodal explants was studied during treatment with growth regulators. This process represents an important indicator of the accommodation capacity of regenerated plantlets to the ex vitro conditions. The cell multiplication rate was stimulated in Murashige and Skoog medium supplemented with indole-3-butyric acid (...

Multiple shoots were obtained from nodal segments of mature trees of Elaeagnus angustifolia L. cultured on MS medium (Murashige and Skoog 1962) supplemented with 0, 0.88 or 2.22 micro M N(6)-benzyladenine. When nodal segments taken from the in vitro proliferated shoots were cultured under the same conditions, additional multiple shoots were obtained. Rooting of the in vitro propagated shoots wa...

Pears are among the most economically important fruits in world that grown all temperate zones. Pyrus communis L., ‘Pyrodwarf®(S)’ rootstock is one of gene sources used to improve fruit productivity, resistance, and tolerance biotic abiotic stresses. Traditional propagation P. L. time-consuming limited by a short growing season harsh winter conditions. Therefore, vitro suitable alternative. Mur...