Vasicine (1) was isolated from the ethanolic extract of Adhatoda vasica Nees (Acanthaceae) and the structure was confirmed using spectroscopic techniques. Acetylcholine esterase, trypsin, DPPH inhibition potential and FRAP assay were carried out using in vitro models. The results showed 38.4 ± 1.2% and 37.4 ± 1.1% activity in acetylcholine and trypsin inhibition assays respectively. The compoun...

This paper describes the development of a novel microfluorimetric assay to measure the inhibition of Plasmodium falciparum based on the detection of parasitic DNA by intercalation with PicoGreen. The method was used to determine parasite inhibition profiles and 50% inhibitory concentration values of known or potential antimalarial drugs. Values for parasite inhibition with known anti-malarial d...

Thiosemiccarbozones are having enormous medicine applications due to the presence of hetero atom nitrogen and sulfur in its molecular structure. The carbonyl compounds also largest clinical cure many diseases. We merged these two classes organic their substitution by solution growth techniques crystal tested as antibiotic against few fastidious non gram +ve –ve organisms. Ortho substituted para...

Abstract In the present work, evaluation of use Trichoderma spp. for controlling different phytopathogens agricultural importance ( Fusarium oxysporum , Rhizoctonia solani Sclerotinia sclerotiorum Botrytis cinerea Colletotrichum gloeosporioides and Phytophthora sp.) was carried out. Confrontation assays by dual culture, volatile metabolites (overlapping plate assay), diffusible (poison medium a...

Poly(ADP-ribose)polymerase (PARP) is a significant therapeutic target for the treatment of numerous human diseases. Olaparib has been approved as PARP inhibitor. In this paper, series new compounds were designed and synthesized with lead compound. order to evaluate inhibitory activities against PARP1 compounds, in vitro inhibition assay intracellular PARylation conducted. The results showed tha...

The fluorometric neuraminidase assay method using 4-methylumbelliferyl-N-Ac-aD-neuraminide as a substrate was investigated for the purpose of ascertaining its validity as a procedure for influenza viral neuraminidase antibody inhibition test. The enzyme antibody inhibition titer obtained by the fluorometric assay system was far weaker than that obtained by the standard colorimetric neuraminidas...

Antioxidant activity of Berry (Morus alba var. nigra) was investigated. For superoxide anion radical assay, the superoxide anion radicals were generated by a pyrogallol auto oxidation system, Nitric oxide radical inhibition by the use of Griess Illosvoy reaction and reducing power was determined according to the Oyaizu method. At least this sample was represented a potential antioxidant capacit...

The ribosome inactivating proteins (RIPs) are a group of proteins that are able to inactivate eukaryotic protein synthesis by attacking the 28S ribosomal RNA. Recent studies have shown that some RIPs possess strong anti-human immunodeficiency virus (HIV) activity. In this study, several common plant RIPs including agrostin, gelonin, luffin, alpha-momorcharin, beta-momorcharin, saporin and trich...

is known as toothache plant belongs to the family of Asteraceae. It treated a medicinal remedy like tuberculosis, illness, cough, rheumatism, and illness. This study was concentrated on antioxidant, cytotoxic, antimicrobial & thrombolytic activities methanol extract leaves using in vitro model. The antioxidant activity estimated trolox equivalent capacity utilizing DPPH reducing power tests...

abstract stachys is one of the largest genera in the flowering plant family. it is in the subfamily lamioideae (labiatae), spreading and growing in different parts of iran. the purpose of this study was to investigate the chemical properties of stachyslavandulifolia as well as the essence, ethanol and methanol extracts of this plant. it was collected from chulus located in the west of mazandara...