ABSTRACT Apoptosis and inflammation were the main hallmarks of sepsis-induced cardiomyopathy (SIC). Yes-associated protein isoform 1 (Yap1) miR-484 involved in mitochondrial fission apoptosis, especially proapoptotic roles SIC. Here, we investigated role Yap1 lipopolysaccharide (LPS)-treated H9c2 cells. was downregulated, while elevated by LPS treatment. Cell counting kit-8, flow cytometry, wes...

Purpose: To investigate the effect of excisanin A on human hepatocellular carcinoma cells as well to elucidate its mechanism action.
 Methods: Molecular docking was used determine binding characteristics HIF-1α protein. The transcriptional activation and viability were assessed using Luciferase reporter MTT assay. protein in nucleus assayed western blot immunofluorescence. VEGF mRNA levels...

MOTIVATION Protein-protein interactions (PPIs) are a promising, but challenging target for pharmaceutical intervention. One approach for addressing these difficult targets is the rational design of small-molecule inhibitors that mimic the chemical and physical properties of small clusters of key residues at the protein-protein interface. The identification of appropriate clusters of interface r...

The dehydrogenase, FrsC, which catalyzes the formation of l-phenyllactate from phenylpyruvate was characterized in this work. enzyme has an extended substrate loop and adapted to accommodate aromatic substrates. Phenyllactate is essential component potent specific G-protein inhibitor natural product FR900359, produced by soil bacterium Chromobacterium vaccinii (photo credit: Eye Science). More ...

Cefiderocol is a cephalosporin designed to treat multidrug-resistant Gram-negative infections. By forming chelated complex with ferric iron, cefiderocol transported into the periplasmic space via bacterial iron transport systems and primarily binds penicillin-binding protein 3 (PBP3) inhibit peptidoglycan synthesis. This mode of action results in having greater vitro activity against many bacil...

the life cycle of the american trypanosome, trypanosoma rangeli, in its invertebrate host, rhodnius prolixus, is completed by invasion into the insect salivary glands. as a prerequisite, there is an initial recognition of the salivary gland receptors for the adhesion/invasion by t rangeli. therefore, the purpose of this study was to investigate the putative receptor molecules on either the sali...

sesame oil cake (ssc) was bio-processed through solid state fermentation (ssf) under optimized conditions by a phytase-producing fish gut bacterium, bacillus subtilis subsp. subtilis (jx292128). ssf significantly reduced anti-nutritional factors (e.g., phytic acid, tannins and trypsin inhibitor) and crude fibre, while enhanced free amino acids, fatty acids and different minerals. phytase product...

A heat-stable protein inhibitor of the hydroxymethylglutaryl-CoA reductase phosphatase 2A activity has been identified and purified to homogeneity, as judged by polyacrylamide gel electrophoresis. The apparent molecular mass was 20,000 Da. The protein lost its inhibitory properties when incubated with trypsin or treated with ethanol. The inhibitor protein does not inhibit type 1 phosphatase whe...

nano-films consisting of an alternating sequence of positively and negatively charged polyelectrolyteshave been prepared by means of the electrostatic layer-by-layer sequential assembly technique on mildsteels. the mild steels were pretreated electrochemically to modify the mild steel surface. the modificationof the mild steel surface resulted in increasing the adhesion of the obtained nano-fil...