Ehrlich ascites tumor cell extracts form a gel when warmed to 25 degrees C at pH 7.0 in sucrose solution, and the gel rapidly becomes a sol when cooled to 0 degrees C. This gel-sol transformation was studied quantitatively by determining the volume or the total protein of pellets of gel obtained by low-speed centrifugation. The gelation depended on nucleotide triphosphates, Mg2+, KCl, and a red...

Silk fibroin (SF) is a typical fibrous protein that is secreted by silkworms and spiders. It has been used in a variety of areas, and especially for tissue-engineering scaffolds, due to its sound processability, mechanical properties, biodegradability, and biocompatibility. With respect to gelation, the SF gelation time is long in aqueous solutions, so a novel approach is needed to shorten this...

high purity fluorapatite (fa) with rod-like and spherical-like morphology was synthesized via sol-gel method. chemical characterization of fa powders was done by xrd and ftir analyses. crystallite samples were calculated using scherer method. morphology of fa powders was investigated with tem and sem images. the results revealed that increasing the time of hydrolysis of phosphate sols significa...

in recent years, chitosan nanoparticles have been studied widely for protein delivery. in this study, hemiscorpius lepturus (hl) venom was encapsulated in chitosan nanoparticles. the aim of the present work was to carry out a systematic study for preparing biocompatible and biodegradable nanoparticles for loading hl scorpion venom and to evaluate their potential as an antigen delivery system. i...

PURPOSE To study the in vitro and in vivo interaction of chitosan nanoparticles (CSNPs), a new particulate drug carrier, with epithelial cells on the ocular surface. METHODS CSNPs labeled with fluorescein isothiocyanate-bovine serum albumin were produced by ionotropic gelation. Human conjunctival epithelial cells (IOBA-NHC) were exposed for 15, 30, 60, and 120 minutes to three different CSNP ...

Floating calcium alginate beads based on gas-generation technique often suffer from poor drug encapsulation and rapid drug release in the acidic environment of the stomach. To overcome the above mentioned demerits, in the present study we have evaluated the influence of formulation factors such as incorporation of mixtures of gasgenerating agents (NaHCO3 and CaCO3), incorporation of gelatin typ...

Long-term (multiple-week or -month) release of small, water-soluble molecules from hydrogels remains a significant pharmaceutical challenge, which is typically overcome at the expense of more-complicated drug carrier designs. Such approaches are payload-specific and include covalent conjugation of drugs to base materials or incorporation of micro- and nanoparticles. As a simpler alternative, he...

Microspheres (MS) of Ketorolac Tromethamine (KT) for oral delivery were prepared by complex coacervation (method-1) and simple coacervation (method-2) methods without the use of chemical crossâlinking agent (glutaraldehyde) to avoid the toxic reactions and other undesirable effects of the chemical cross-linking agents. Alternatively, ionotropic gelation was employed by using sodium-tripolyphosp...

Retinyl palmitate (RP)-loaded pectinate micro- and nano-particles (PMP and PNP) were designed for stabilization of RP that is widely used as an anti-wrinkle agent in anti-aging cosmeceuticals. PMP/PNP were prepared with an ionotropic gelation method, and anti-oxidative activity of the particles was measured with a DPPH assay. The stability of RP in the particles along with pectin gel and ethano...

Purpose: To formulate rifabutin-loaded floating gel beads for stomach-specific release. Methods: Rifabutin-loaded floating gellan gum beads were prepared by ionotropic calcium-induced gelation in acidic medium. In-vitro buoyancy and drug release studies were performed using a USP dissolution apparatus type II in 0.01M HCl (ph 2.0) as the dissolution medium. The shape, surface morphology and int...