Immunofixation following electrophoresis or isoelectric focusing has proven to be a powerful tool for the phenotyping of plasma proteins, the identification of proteins such as M-components and the study of conversion or breakdown products of proteins such as C3. One of the major advantages is the ability to compare, side by side, the mobilities of relatively low-concentration proteins. The tec...

A new method called dynamic kinetic capillary isoelectric focusing (DK-CIEF) is presented for the study of protein-DNA interactions. The method is based on CIEF with laser-induced fluorescence-whole column imaging detection in which protein-DNA complexes are separated with spatial resolution while dissociations of the complexes are dynamically monitored using a CCD camera with temporal resoluti...

Conalbumin complexes containing one, and only one, specifically bound metal ion were isolated by the isoelectric focusing technique. This made possible the preparation of a series of mixed metal or mixed isotope complexes: GaoFeiconalbumin, Fe,Gaiconalbumin, S6Fezconalbumin, 5Te05Teiconalbumin, “FeoS6Feiconalbumin, and 57Fezconalbumin. The appropriate species were studied by EPR, Mijssbauer, an...