Glutamate (Glu) dehydrogenase (GDH, EC 1.4.1.2-1.4.1.4) catalyzes in vitro the reversible amination of 2-oxoglutarate to Glu. The in vivo direction(s) of the GDH reaction in higher plants and hence the role(s) of this enzyme is unclear, a situation confounded by the existence of isoenzymes comprised totally of either GDH beta- (isoenzyme 1) or alpha- (isoenzyme 7) subunits, as well as another f...

A 65-year-old woman failed to develop increased creatine kinase or lactate dehydrogenase activity after a myocardial infarction. She had no measurable creatine kinase MB isoenzyme and no detectable patterns of normal LD isoenzyme activity. These determinations five months after the infarction showed normal values for total activity and isoenzyme patterns.

We describe an atypical serum creatine kinase isoenzyme in the serum of a woman with cancer of the left breast. This isoenzyme migrated toward the cathode, closely following the MM isoenzyme on agarose gel electrophoresis. Its relative molecular mass was estimated to be about 325,000, fourfold that of normal creatine kinase. It is more heat-stable and is inhibited more by urea than the normal M...

In vitro incubation, at 37 degrees C, of human creatine kinase isoenzyme-1 (isoenzyme BB) and human immunoglobulin G in a buffer results in the formation of a complex of high relative molecular mass (Mr approximately 825,000), which contains both proteins. This complex also forms in vitro if creatine kinase isoenzyme-1 is incubated with fresh human serum. The creatine kinase activity of the com...

plantation of fast-growing species such as poplar is one of the important methods for wood production in the countries with low natural forest areas. physiological differences of populus deltoides clones planted through three decades in shast kalate research forest are unknown. peroxidase enzyme in this research was used for clone separation as a biochemical marker that is sensitive to environm...

要 旨:エ ナメル上皮腫,歯 肉癌 および唾液腺腫瘍 の各種 口腔領 域上皮性腫瘍組織 につ いて,DNA量 あた りのLDH活 性 とLDH isoenzyme patternを 正常対 照組織 と比較 して検討 した。 LDH活 性 は,Wroblewski and LaDueの 方 法に準 じてNADHの 吸光度変化 に よ り測 定 した。LDH isoenzyme patternは,Dietz and Lubranoの 方 法に準 じてdisc電 気泳動 によ り分離 し,Ressler氏 法の 変法 による染色液 で酵素活性 を検 出 しdensitometryに よ り求 めた。 その結果,一 般 に腫瘍組織 においてはLDH活 性の上昇が認 め られ た。 エナメル上皮腫 のLDHisoenzyme patternはLDH-3にpeakが み られ,仔 牛 エナメル器...

The effect of gastrointestinal surgery on serum creatine kinase activity was studied in 30 patients. The MB isoenzyme was demonstrated in sera of 30% of the patients and BB isoenzyme in 23%. MB content varied from 0.8 to 10.3% of the total creatine kinase activity, and the BB content from 0.6 to 18.4%. The CK-BB was probably of gastrointestinal origin, since gastrointestinal tract contains high...

We assessed the reported presence of creatine kinase-BB isoenzyme in the serum of patients with chronic renal failure. Our study showed that the blood of these patients contains a fluorescent material with an electrophoretic mobility similar to that of the BB isoenzyme on cellulose acetate strips. The fluorescing material is, however, completely distinct from BB isoenzyme and its nature is as y...