The net synthesis of low density plasma lipoproteins has been shown in the perfused rat liver and in rat liver slices in work reported from this laboratory (2). Haft, Roheim, White, and Eder (3) demonstrated amino acid incorporation into low and high density plasma lipoproteins in the perfused liver, and Radding and Steinberg (4) reported similar observations in rat liver slices. The present ex...

The pathogenesis of liver disease in protoporphyria has been presumed to result from the hepatic deposition of protoporphyrin. To examine the effects of protoporphyrin on hepatic bile flow and histopathology, studies were performed employing an isolated, in situ, rat liver perfusion system. Rat livers in the control group were perfused with 0-80 mumol sodium taurocholate/h. Rat livers in the ex...

1. The relationship between extracellular pH (pH,), intracellular pH (pH,) and lactate uptake was studied in the isolated perfused rat liver during simulated metabolic acidosis. 2. pHi fell to a considerably less extent than pH, when the latter was decreased from pH 7.4 to 6.7. 3. The liver took up lactate when pHi was greater than 7.0; at lower values of pH, lactate output occurred. 4. The rel...

The liver is capable of storing large quantities of vitamin A in ester form (1). However, some uncertainty still exists concerning both the composition of liver vit,amin A ester and the ability of the liver to esterify vitamin A. Vitamin A palmitate was isolated from fish liver many years ago (a), and recently it has been suggested that this ester is the principal if not exclusive (3, 4) ester ...