The spatial relationships between the protein constituents to two junctional structures, adhaerens junctions and desmosomes, were determined by double immunofluorescence microscopy using marker proteins specific for these structures. Adhaerens junctions were visualized by immunofluorescent labeling for the membrane-associated protein vinculin and by their association with actin filaments. Desmo...

Abstract Objectives Dentifrices containing zinc reduce gingival inflammation and bleeding better than control dentifrices (no zinc). How might work is not understood. We have shown that lysine decarboxylase (LdcE), an enzyme from Eikenella corrodens, converts to cadaverine in dental biofilms. The lack of impairs the dentally attached cell barrier biofilm, causing biofilm products leak into junc...

The Hippo pathway, by tightly controlling the phosphorylation state and activity of the transcription cofactors YAP and TAZ is essential during development and tissue homeostasis whereas its deregulation may lead to cancer. Recent studies have linked the apicobasal polarity machinery in epithelial cells to components of the Hippo pathway and YAP and TAZ themselves. However the molecular mechani...

Objective: Junctional ectopic tachycardia is common after cardiac surgery for congenital heart disease. However, its incidence and related risk factors in infants are not well known. The objective of this study was to determine the overall junctional neonates infants. Methods: We enrolled a total 271 patients aged

A dense plexus of CGRP-immunoreactive nerve fibers was found subjacent to and within the epithelium of the excretory ducts of the parotid, submandibular and sublingual glands in the rat, in close proximity to the opening into the oral cavity. The immunoreactive nerve fibers disappeared after a neonatal administration of capsaicin. In immuno-electron microscopy, the immunoreactive nerve fibers w...

The structural features of the chloride-secreting opercular epithelium of Seawateradapted killifish (Fundulus heteroclitus) were examined by thin-section and freeze-fracture electron microscopy, with particular emphasis on the morphological appearance of occluding junctions. This epithelium is a flat sheet consisting predominantly of groups of mitochondriarich chloride cells with their apices a...

background: the aim of the present study was to evaluate the efficacy of autologous serum eye drop in treatment of recurrent corneal epithelial defect. m aterials and methods: fourteen patients with recurrent corneal epithelial defect were studied. autologous serum was prepared from the patients and diluted in 20% normal saline. the patients were instructed to use the autologous serum every six...

Lens epithelial cells communicate with two different cell types. They communicate with other epithelial cells via gap junctions on their lateral membranes, and with fiber cells via junctions on their apices. We tested independently these two routes of cell-cell communication to determine if treatment with a 90% CO2-equilibrated medium caused a decrease in junctional permeability; the transfer o...

The morphology of the tight junction of rat thyroid epithelium was examined in freeze-fractured material fixed in glutaraldehyde and briefly glycerinated. In normal thyroids the overall appearance of this junctional specialization resembled that of other cell types in many respects. Short-term changes in thyroid activity and hypophysectomy for 3 wk did not obviously affect the appearance of t...