This study aimed to compare the sensitivity of multiplex PCR to ELISA technique in the instantaneous detection of HBV, HCV and HIVin blood samples from donors of the National blood Transfusion Centre in Togo. A total of 440 blood samplesfrom volunteer were collected and tested by ELISA and multiplex PCR for HBV, HCV and HIV detection. Among the 440 volunteer blood donors, 83% were female and 17...

background and aims: infectious mononucleosis (im)is the clinical manifestation of primary infection with epstein-barr virus (ebv). humans are the only known reservoir of ebv. regarding the problems in diagnosis of the disease, the purpose of this study was to assess enzyme-linked immunosorbent assay (elisa) and nested polymerase chain reaction (pcr) as a diagnostic tool for this disease. mater...

background : this comprehensive study was conducted on multi-purpose one-humped camel ( camelus dromedarius ) sera and ticks to assess the epidemiological aspects of the crimean-congo hemorrhagic fever virus (cchfv) in northeast iran. methods : from may 2012 to january 2013, eleven cities were randomly selected in the khorasan provinces of iran as “clusters,” and at least 14 one-humped camels w...

avian leukosis viruses (alvs) cause different types of tumours in poultry and can affect the health and egg production of the birds. to investigate the presence of the virus in chicken layer flocks in shiraz, 222 egg albumen from local layer breeder (25 eggs), local layer grand parent (30 eggs), broiler breeder (60 eggs), commercial layer (46 eggs) and broiler grand parent (61 eggs) were tested...

background there are several conventional, immunological and molecular techniques to diagnose the fungi that cause aspergillosis in biological samples; these methods have some advantages and disadvantages. objectives the current study aimed to evaluate different methods used in identification and diagnosis of fungi causing aspergillosis. materials and methods male western albino rats were provi...

abstract beet yellows virus (byv), the type member of the genus closterovirus and one of the most economically important yellowing viruses, causes serious losses in sugarbeet. in order to study infection and distribution of beet yellows virus during summer 2007, about 530 samples were collected from beet’s fields in razavi khorasan province (fariman, torbate jam, ghoochan, chenaran, torbate hey...

background: visceral leishmaniasis (vl) is caused by leishmania infantum in mediterranean basin and is an endemic disease in some parts of iran. canines are the main reservoirs of vl in most of the endemic areas. different serological methods have been introduced for diagnosis of canine visceral leishmaniasis (cvl). objective: in this survey a fucose-mannose ligand (fml) elisa, using native l. ...

zucchini yellow mosaic virus (zymv) is a potyvirus with a worldwide distribution. this virus causes serious economic losses in iran in many cucurbits. during 2002-2003, sam-ples were collected from squash fields in tehran province. five isolates (z1, z2, z3, z4 and z5) were inoculated on 27 species of cucurbitaceae, chenopodiaceae, amarantha-ceae,solanacae, leguminosae and ranunculaceae. chenop...