نتایج جستجو برای: keywords aptamer

تعداد نتایج: 1982596  

2015
Francis Doyle Seung Soo Oh Jeff Nie Ron Stewart Peter J. Coffey James A. Thomson H. Tom Soh Minseon Cho

Array-based Discovery of Aptamer Pairs Report Title Affinity reagent pairs that recognize distinct epitopes on a target protein can greatly improve the sensitivity and specificity of molecular detection. Importantly, such pairs can be conjugated to generate reagents that achieve twosite “bidentate” target recognition, with affinities greatly exceeding either monovalent component. DNA aptamers a...

2018
Ewa Kowalska Filip Bartnicki Ryo Fujisawa Piotr Bonarek Paweł Hermanowicz Toshiki Tsurimoto Klaudia Muszyńska Wojciech Strzalka

Proliferating cell nuclear antigen (PCNA) is a multifunctional protein present in the nuclei of eukaryotic cells that plays an important role as a component of the DNA replication machinery, as well as DNA repair systems. PCNA was recently proposed as a potential non-oncogenic target for anti-cancer therapy. In this study, using the Systematic Evolution of Ligands by EXponential enrichment (SEL...

Journal: :International Journal of Molecular Sciences 2008
Laura Bonifacio Frank C. Church Michael B. Jarstfer

Here we tested the ability to augment the biological activity of the thrombin aptamer, d(GGTTGGTGTGGTTGG), by using locked nucleic acid (LNA) to influence its G-quadruplex structure. Compared to un-substituted control aptamer, LNA-containing aptamers displayed varying degrees of thrombin inhibition. Aptamers with LNA substituted in either positions G5, T7, or G8 showed decreased thrombin inhibi...

2014
Hongguang Sun Xun Zhu Patrick Y Lu Roberto R Rosato Wen Tan Youli Zu

Aptamers are a class of small nucleic acid ligands that are composed of RNA or single-stranded DNA oligonucleotides and have high specificity and affinity for their targets. Similar to antibodies, aptamers interact with their targets by recognizing a specific three-dimensional structure and are thus termed "chemical antibodies." In contrast to protein antibodies, aptamers offer unique chemical ...

Journal: :Chemical communications 2014
Timothy R Pearce Brett Waybrant Efrosini Kokkoli

The self-assembly of single-stranded DNA (ssDNA) aptamer-amphiphiles was influenced by the choice of spacer used to link the hydrophobic tail and aptamer headgroup. Aptamer-amphiphiles without spacers or with hydrophilic spacers formed globular micelles while amphiphiles with hydrophobic spacers formed bilayer nanotapes, which are the first such structures formed by DNA-amphiphiles.

Journal: :The Analyst 2012
Gergely Lautner Zsófia Balogh Anna Gyurkovics Róbert E Gyurcsányi Tamás Mészáros

We introduce Amplified Luminescent Proximity Homogenous Assay (ALPHA) to assess the K(D) value of aptamer-protein complexes as demonstrated through the study of apple stem pitting virus coat protein-specific aptamers. This method can be used as a simple, cost-effective method for screening aptamer-target protein interactions during aptamer selection.

Journal: :Current Opinion in Electrochemistry 2021

Aptamer-based electrochemical sensors are now developed for the detection of a wide variety analytes including ions, low-molecular-weight molecules, proteins, and living cells. An aptamer-based sensor is an analytical device whose bio-sensing element (i.e. aptamer) immobilized on transducer surface. Aptasensors have attracted great attention because their high selectivity, sensitivity, stabilit...

Journal: :Nucleic acids research. Supplement 2003
Yoshiharu Hayashi Satoshi Takahashi Katsuyoshi Sakaguchi Nao Iwata Masaki Kobayashi

In this paper, we introduce a system named Comparative Molecular Interaction Profile Analysis system (CoMIPA) that utilizes virtual docking study for comprehensive analysis of molecular interactions. Research of RNA aptamer is very useful to creation of functional molecules, because RNA aptamer is excellent in binding selectivity and the stability of structure. By using 3-bases RNA fragment, th...

Journal: :Journal of the Medical Library Association : JMLA 2015

Journal: :Academic Medicine 1990

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