Reverse transcription (RT) followed by polymerase chain reaction (PCR) is the technique of choice for analysing mRNA in extremely low abundance. Real-time RT-PCR using SYBR Green I detection combines the ease and necessary exactness to be able to produce reliable as well as rapid results. To obtain highly accurate and reliable results in a real-time RT-PCR a highly defined calibration curve is ...

We developed a new homogeneous assay-using SYBR Green I and repeats of 20 C nucleotides-for the highly selective and sensitive detection of silver ions and silver nanoparticles.

Abstract Background To make the right treatment decisions about colorectal cancer (CRC) patients reliable predictive and prognostic data are needed. However, in many cases this is not enough. Some studies suggest that LRIG1 gene ( leucine-rich repeats immunoglobulin-like domains1 ) has implications different kinds of cancers. Methods One hundred two with were retrospectively analyzed for expres...

A simple, rapid, and signal-on fluorescent assay was developed for activity analysis of DNA methyltransferase and for screening of its inhibitors based on a methylation-resistant endonuclease and SYBR Green I.

Combining a DNA intercalator, SYBR Green I, and enzyme-linkage reactions with carbon nanoparticles, a low-background biosensing platform for label-free and sensitive fluorescent assay of DNA methylation is reported.