A novel cDNA encoding alpha subunit of the GTP-binding protein, transducin, has been cloned from a marine fish, Sparus aurata. The cDNA contains an open reading frame of 1050 nt (encoding 350 amino acid residues). A high degree of identity was found with known mammalian transducin proteins of cones (Gt2 alpha) or rods (Gt1 alpha): human Gt2 alpha (80.2%), bovine Gt2 alpha (79.3%), mouse Tt1 alp...

Some S. aurata (one wild and two farmed fish) populations namely, (D, A & K21) were genetically characterized using some DNA markers. The homogeneity value was high within each estimated fish population. The genetic dissimilarity values were calculated for reconstruction the dendrogram among them. The (D) population was distantly related from (A & K21) populations. The results suggested that, t...

objective: changes in chloride cell abundance, na+, k+-atpase immunolocalization and activity were investigated in the gills of the golden grey mullet, liza aurata, fry acclimated to freshwater (fw) and different salinities (12‰, 36‰ and 46‰). materials and methods: na+, k+-atpase localization was performed through immunofluorescence light microscopy using a mouse monoclonal antibody iggα5. qua...

We announce the draft genome sequences of Vibrio alginolyticus strains V1 and V2, isolated from juvenile Sparus aurata and Dentex dentex, respectively, during outbreaks of vibriosis. The genome sequences are 5,257,950 bp with a G+C content of 44.5% for V. alginolyticus V1 and 5,068,299 bp with a G+C content of 44.8% for strain V2. These genomes provide further insights into the putative virulen...

A soluble fructose-1,6-bisphosphate aldolase enzyme has been purified 50.2-fold (2.36%) at the homogeneity from the electric organ of Electrophorus electricus by one step of DEAE-52 anion exchange chromatography followed by Superose-12 gel filtration-FPLC. Like other aldolase enzymes the E. electricus protein is a dimer with two identical subunits of 45 kDa. The N-terminal (20 residues) reveale...

Despite the Antarctic Ocean being considered a pristine environment, elevated trace element concentrations have been reported in many marine organisms. The is particularly vulnerable to climate change, which can also affect bioaccumulation of biota. While octopods are key components regional food webs as prey for variety predators (e.g., seals, fish, and seabirds), their contamination state by ...