To elucidate the mechanisms leading to the functional regionalization of the digestive epithelium, lactase expression was analyzed at the protein, mRNA and gene levels, along the intestinal tract at various stages of the rat postnatal development. In the colon of neonates, the transient expression of mRNA and enzyme correlated well with gene transcription. In contrast to the colon, complex patt...

Lactase-phlorizin hydrolase complex isolated from the membrane fraction of the small intestine of Z-week-old rats has a glycosylceramidase activity similar to that reported by Brady et al. ((1965) J. BioZ. Chem. 240, 3766). The glycosylceramidase activity corresponds to phlorizin hydrolase rather than to lactase. The “physiological” substrates of small intestinal phlorizin hydrolase (the activi...

Lactose is a prominent sugar found in all diary products, as well as in mother's milk. Lactose maldigestion/intolerance is the term used to describe the inability to digest lactose in milk and milk products, producing symptoms of intestinal gas, abdominal distension, and flatulence with loose, watery, acidic diarrhea.Adverse reactions to the lactose portion of milk are primarily related to the ...

Two hundred and ninety four duodenal and jejunal mucosal biopsy specimens from patients with coeliac disease, treated and untreated, and other conditions were examined histologically and by histochemical staining for five peptidase and three disaccharidase enzymes to determine profiles of activity. Suppression of activity paralleled the histology with the following enzymes: lactase, trehalase, ...

Lactase-phlorizin hydrolase complex isolated from the membrane fraction of the small intestine of Z-week-old rats has a glycosylceramidase activity similar to that reported by Brady et al. ((1965) J. BioZ. Chem. 240, 3766). The glycosylceramidase activity corresponds to phlorizin hydrolase rather than to lactase. The “physiological” substrates of small intestinal phlorizin hydrolase (the activi...

Background: Disaccharidase (DS) deficiencies have been reported in pediatric patients with inflammatory bowel disease (IBD), but the relationship between duodenal inflammation and DS deficiency has not evaluated outside of lactase deficiency. Methods: This study assessed levels IBD who underwent endoscopy assessment activity. Records were reviewed for subtype, pathology findings, results analys...

Duodenal mucosa showed normal morphology, interepithelial lymphocytes, alkaline phosphatase, and sucrase in a girl with growth retardation and iron deficiency, but normal absorption of lactose and xylose after two years of abnormal stools. Mucosal lactase was low. Fourteen months later mucosal damage consistent with coeliac disease was evident, and gluten intolerance was subsequently confirmed ...

Lactase activity in presence of mono and divalent metal ions, as its cofactors, and some reducing agents was studied. Activation effects of k and Mn were better than other cations and dithiotrethiol was more inhibitor. By inhibition constant, 12.15mMol, the type of inhibition was non-competitive. The kinetic model of Lactase hydrolysis reaction also was determined. Among glucose and galactose a...