Mammalian sperm undergomultiple maturation steps after leaving the testis in order to become competent for fertilization, but the molecular mechanisms underlying this process remain unclear. In terms of identifying factors crucial for these processes in vivo, we found that lipocalin 2 (Lcn2), which is knownas an innate immune factor inhibiting bacterial and malarial growth, can modulate sperm m...

Immune fibroblastic cell (IFC) subpopulations of dermal fibroblasts differentiate into adipocytes and provide skin immune defense in response to infection. To better understand the role these fibroblast subsets, we have undertaken a systematic analysis models inflammation. First, communication from mesenchymal, epithelial, bone-marrow derived cells was investigated using unbiased machine learni...

Due to the heterogeneous nature of breast cancer, metastasis organotropism has been poorly understood. This study assessed specific cancer-related gene expression changes occurring with metastatic cancer recurrence distant organs compared non-metastatic cancer. We found that several secreted mediators encoding genes notably, LCN2 and S100A8 overexpressed at site spine (LCN2, 5-fold; S100A8, 6-f...

We validated a single-stranded, DNA aptamer-based, diagnostic method capable of detecting Lipocalin-2 (LCN2), a biomarker from clinically relevant hepatocellular carcinoma (HCC) patient serum, in the sandwich assay format. Nine aptamers (LCN2_apta1 to LCN2_apta9) for LCN2 were screened with SELEX processes, and a sandwich pair (LCN2_apta2 and LCN2_apta4) was finally chosen using surface plasmon...

Endothelial cells are spatially close to osteoblasts and regulate osteogenesis. Moreover, they are sensitive to mechanical stimuli, therefore we hypothesized that they are implicated in the regulation of bone metabolism during unloading. Conditioned media from endothelial cells (EC-CM) subjected to simulated microgravity (0.08g and 0.008g) increased osteoblast proliferation and decreased their ...

The tumor suppressor gene HIC1 is frequently deleted or epigenetically silenced in human cancer, where its restoration may improve cancer prognosis. Here, we report results illuminating how HIC1 silencing alters effect or signals in triple-negative breast cancer (TNBC), which are crucial for its pathogenesis. HIC1 expression was silenced only in TNBC compared with other molecular subtypes of br...

Background: Performing sports activities along with nutritional interventions is recommended as an effective way to reduce inflammatory cytokines and improve insulin resistance in obese people. This study aimed evaluate the effect of aerobic exercise Ginkgo biloba herbal supplementation on lipocalin 2 (Lcn2) levels men. Materials Methods: For this purpose, 40 men Kashan were selected accessible...

The human 25-kDa Lipocalin 2 (LCN2) was first identified and purified as a protein that in part is associated with gelatinase from neutrophils. This shows high degree of sequence similarity the deduced sequences rat α -microglobulin-related mouse 24p3. Based on its typical lipocalin fold, which consists an eight-stranded, anti-parallel, symmetrical β-barrel fold structure it initially thought L...

OBJECTIVE The proinflammatory cytokines/adipokines produced from adipose tissue act in an autocrine and/or endocrine manner to perpetuate local inflammation and to induce peripheral insulin resistance. The present study investigates whether lipocalin-2 deficiency or replenishment with this adipokine has any impact on systemic insulin sensitivity and the underlying mechanisms. METHODS AND RESU...