نتایج جستجو برای: linked immunospot assay
تعداد نتایج: 428010 فیلتر نتایج به سال:
The ELISPOT assay is considered by many to be a gold standard for monitoring specific cellular immune responses. It can detect single cells secreting molecules of interest (1-3). This assay is highly sensitive, quantitative, easy to use and amenable to high throughput (4). Until recently assays using chromophores to detect the secretion of cytokines from single cells was limited by the difficul...
CD8 T-cell responses were examined in subjects with incident (new following negative visits) or prevalent (lasting ≥ 4 months) human papillomavirus type 16 (HPV16) or human papillomavirus (HPV18) infection. The groups were chosen from a cohort of women being followed every 4 months with cervical cytology and HPV-DNA testing. Enzyme-linked immunospot (ELISPOT) assay was performed at enrollment (...
The interferon gamma, enzyme-linked immunospot (IFN-γ ELISpot) assay is widely used to identify viral antigen-specific T cells is frequently employed to quantify T cell responses in HIV vaccine studies. It can be used to define T cell epitope specificities using panels of peptide antigens, but with sample and cost constraints there is a critical need to improve the efficiency of epitope mapping...
BACKGROUND Very little is known about the association between infection with Toxoplasma gondii (T. gondii) and diabetes mellitus. We perform an age- and gender-matched case-control study to determine the association of T. gondii infection and diabetes mellitus. METHODS Cases included 156 patients with diabetes mellitus and 156 controls without diabetes mellitus who attended in two public clin...
Materials and methods: One group of pigs administered concurrently Fostera PCV and Fostera PRRS (Zoetis, Florham Park, New Jersey) and another group administered concurrently Ingelvac CircoFLEX and Ingelvac PRRS MLV (Boehringer Ingelheim Vetmedica Inc, St Joseph, Missouri) at study day -28 (21 days of age) were challenged with both viruses at study day 0 (49 days of age). Serum samples were tes...
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