The lipase production ability of a newly isolated Acinetobacter sp. in submerged (SmF) and solid-state (SSF) fermentations was evaluated. The results demonstrated this strain as one of the rare bacterium, which is able to grow and produce lipase in SSF even more than SmF. Coconut oil cake as a cheap agroindustrial residue was employed as the solid substrate. The lipase production was optimized ...

An extra-cellular lipase produced by Bacillus licheniformis MTCC 6824 was purified to homogeneity by ammonium sulphate fractionation, ethanol/ether precipitation, dialysis, followed by anion-exchange chromatography on Amberlite IRA 410 (Cl(-) form) and gel exclusion chromatography on Sephadex G 100 using Tris-HCl buffer (pH 8.0). The crude lipase extract had an activity of 41.7LU/ml of culture ...

Lipase was purified from squid (Todarodes pacificus) liver in an attempt to investigate the possibility of applying the enzyme for biotechnological applications. Crude extract of squid liver was initially fractionated by the batch type ion exchange chromatography. The fraction containing lipase activity was further purified with an octyl-Sepharose column. Finally, lipase was purified by eluting...

Biodiesel is one alternative energy that might be promising to substitute the petroleum-based fuel due its biodegradability and less toxic level. Biodiesel can synthesized through non-alcohol route by substituting alcohol with methyl acetate using lipase enzyme as Biocatalyst, which may give by-product during reaction. In this research, from Bacillus subtilis ha...

The presence of NaCl-resistant, neutral triacylglycerol hydrolase (lipase) activity in rat adrenal gland, ovary and testis was studied. Both adrenals and ovaries but not testes were found to contain such a lipase. The activity of the enzyme in the adrenal gland was lowered during cortisol treatment and hypothyroidism. An elevated adrenal lipase activity was found during hyperthyroidism. Pseudo-...

To explore the causes of different growth rates among juvenile populations largemouth bass, in present study, a batch bass juveniles reared under same conditions were divided into fast-growing group and slow-growing group. We used histological, enzymatic molecular biology methods to analyze determine their histomorphological changes, digestive enzyme activity intestinal floral composition. The ...

Marine fungus BTMFW032, isolated from seawater and identified as Aspergillus awamori, was observed to produce an extracellular lipase, which could reduce 92% fat and oil content in the effluent laden with oil. In this study, medium for lipase production under submerged fermentation was optimized statistically employing response surface method toward maximal enzyme production. Medium with soyabe...

Pancreatic lipase is rapidly and irreversibly inactivated at a hexadecane-water interphase. The unfolded protein is only very slowly, in the course of hours or days, released into the aqueous phase. Bile salts prevent the denaturation of the lipase. Bovine albumin also protects the enzyme. Denaturation of lipase occurs also at the interphase of the substrates, tributyrin or olive oil, and water...

Steady state expression of lipoprotein lipase was compared in abdominal and gluteal subcutaneous adipose tissue of nonobese men and women. In both regions enzyme activity and lipoprotein lipase mRNA levels were significantly higher in women than in men. In men the enzyme activity was higher in abdominal than in gluteal adipose tissue (P less than 0.01) whereas the opposite was observed in women...

Pancreatic lipase is rapidly and irreversibly inactivated at a hexadecane-water interphase. The unfolded protein is only very slowly, in the course of hours or days, released into the aqueous phase. Bile salts prevent the denaturation of the lipase. Bovine albumin also protects the enzyme. Denaturation of lipase occurs also at the interphase of the substrates, tributyrin or olive oil, and water...