Oral administration of -polylysine to rats reduced the peak plasma triacylglycerol concentration. In vitro, -polylysine and polylysine strongly inhibited the hydrolysis, by either pancreatic lipase or carboxylester lipase, of trioleoylglycerol (TO) emulsified with phosphatidylcholine (PC) and taurocholate. The -polylysine concentration required for complete inhibition of pancreatic lipase, 10 g...

The lipolytic system of Bacillus megaterium 370 was investigated, showing the existence of at least two secreted lipases and a cell-bound esterase. A gene coding for an extracellular lipase was isolated and cloned in Escherichia coli. The cloned enzyme displayed high activity on short to medium chain length (C4^C8) substrates, and poor activity on C18 substrates. On the basis of amino acid sequ...

Lipids play diverse and important biological roles including maintaining cellular integrity, storing fat for energy, acting as signaling molecules, and forming microdomains to support membrane protein signaling. Altering the levels of specific lipid species through activating or inactivating their biosynthetic or degradative pathways has been shown to provide either therapeutic benefit or cause...

Lk2 and Lk3 were thermostable recombinant lipase highly expressed in Escherichia coli BL21 (DE3). However, accumulated as an inclusion body. To further characterize both lipases, the soluble enzyme must be obtained first. This study aimed to optimize disruption of cell membrane order obtain active lipases. The effects temperature lysis, pH, SDS concentration on lipolytic activity investigated u...