The physiological mechanisms by which growth hormone (somatotropin) exerts its several metabolic activities remain poorly understood. In particular, there is disagreement as to whether the diabetogenic and lipolytic activities of the hormone are intrinsic properties of the molecule or are the result of contamination with other pituitary components. The availability of recombinant-DNA-derived bo...

Free fatty acids are important flavor compounds in cheese. Propionibacterium freudenreichii is the main agent of their release through lipolysis in Swiss cheese. Our aim was to identify the esterase(s) involved in lipolysis by P. freudenreichii. We targeted two previously identified esterases: one secreted esterase, PF#279, and one putative cell wall-anchored esterase, PF#774. To evaluate their...

Bacteriocins are antimicrobial proteins produced by bacteria that inhibit the growth of other bacteria with a bactericidal or bacteriostatic mode of action. Many lactic acid bacteria (LAB) produce a high diversity of different bacteriocins. Bacteriocinogenic LAB are generally recognised as safe (GRAS) and useful to control the frequent development of pathogens and spoilage microorganisms. For t...

A total of 208 strains of lactic acid bacteria (LAB) were isolated from Algerian traditional fermented milk (Jben, Klila, Raib and Lben), in order to evaluate their technological and probiotic properties. These strains were tested for their antimicrobial activity against Listeria monocytogenes using agar well diffusion technique. Only 11 strains were retained, they were also characterised in re...

Pathogenic bacteria secrete various virulence factors, including toxins, lipases and proteases that allow them to infect, breakdown and colonize host tissue. Among various modes of action that the pathogenic bacteria use to damage the host, pore formation (by pore forming toxins (PFTs)) and lipid hydrolysis (by phospholipases) modes are common in damaging the eukaryotic cell membrane. PFTs in t...

Pathogenic bacteria secrete various virulence factors, including toxins, lipases and proteases that allow them to infect, breakdown and colonize host tissue. Among various modes of action that the pathogenic bacteria use to damage the host, pore formation (by pore forming toxins (PFTs)) and lipid hydrolysis (by phospholipases) modes are common in damaging the eukaryotic cell membrane. PFTs in t...

Abstract. Indrayani, Putra RP, Hambali A, Ardiansyah. 2022. Isolation and characterization of extremophile bacteria for hydrolytic enzyme production from Waepella Hot Spring, Sinjai, Indonesia. Biodiversitas 23: 6345-6351. Extremophiles are organisms that have adapted to live in extreme environments. Therefore, they huge potential various industrial applications, specifically production. The ai...

Northeastern Atlantic minke whales (Balaenoptera acutorostrata) have a multichambered stomach system which includes a nonglandular forestomach resembling that of ruminants. Bacteria from the forestomachs of herring-eating whales were enumerated and isolated in an anaerobic rumen-like culture medium (M8W medium). The total viable population of anaerobic bacteria ranged from 73 x 10 to 145 x 10/m...

It has been observed that nucleic acids, nucleotides, and nucleosides inhibit the lipolytic action of epinephrine on adipose tissue, whereas purine and pyrimidine bases increase it (1). Further studies, described in the present report, have shown this interaction to be quite specific for lipolysis (uptake and esterification of fatty acids being unaffected), but not limited to epinephrine (other...

Lipases are enzymes used in various industrial sectors such as food, pharmaceutical and chemical synthesis industries. The selection of microorganisms isolated from soil or wastewater is an alternative to the discovery of new species with high enzymes productivity and with different catalytic activities. In this study, the selection of lipolytic fungi was carried out by submerged fermentation. ...