OBJECTIVE Volume replacement in septic patients improves hemodynamic stability. This effect can reduce the inflammatory response. The objective of this study was to evaluate the effect of 7.5% hypertonic saline solution versus 0.9% normal saline solution for volume replacement during an inflammatory response in endotoxemic rats. METHODS We measured cytokines (serum and gut), nitrite, and lipi...

Millions of molecules of lipopolysaccharide (LPS) must be assembled on the Escherichia coli cell surface each time the cell divides. The biogenesis of LPS requires seven essential lipopolysaccharide transport (Lpt) proteins to move LPS from the inner membrane through the periplasm to the cell surface. However, no intermediate transport states have been observed. We developed methods to observe ...

Uveitis is a major cause of vision loss. Methotrexate (MTX) has been widely used in uveitis due to its relatively safe profile. The purpose of this study was to evaluate the effects of two different dosages of MTX via intra-vitreal administration for treatment of endotoxin induced uveitis (EIU) in an experimental model. Thirty-five healthy rabbits were randomly divided into four groups and all ...

Background and Aims: The therapeutic effects of the olibanum, the resin of Boswellia serrata (B. serrata) from the Burseraceae family in inflammatory disease have been reported. There are more than 200 active ingredients in this resin, including Boswellic acid. It is proposed that aqueous extract of B. serrata can improve memory impairment induced by cerebral inflammation result in the adminis...

Statins have neuroprotective effects on neurological diseases, including a pleiotropic effect possibly related to blood–brain barrier (BBB) function. In this study, we investigated the of pitavastatin (PTV) lipopolysaccharide (LPS)-induced BBB dysfunction in an vitro model comprising cocultured primary mouse brain endothelial cells, pericytes, and astrocytes. LPS (1 ng/mL, 24 h) increased perme...

Objective: To investigate how dihydroartemisinin (DHA) modulate Toll-like receptors-4 signaling pathway in LPS-stimulated RAW264.7 murine. Methods: IRF3 was detected in RAW264.7 cells treated with lipopolysaccharide (LPS) (1 μg/ml), lipopolysaccharide and dihydroartemisinin (LPS/DHA) (10 μM) and equal volume of culture medium respectively by using immunocytochemistry staining. IFN-α/β mRNA was ...

cytotoxic effects of obturation materials were tested in presence and absence of endotoxin on human monocytes in vitro.human monocytes from thp-1 cell line were cultured. three millimeters from the tip of each resilon and gutta percha points were cut and directly placed at the bottom of the culture wells. cultured cells were exposed to gutta percha (groups g1 and g2) and resilon (r1 and r2). te...